Skip to main content Skip to navigation
Hamburger Menu
Search icon
Location Selector Location Selector
Netherlands (English)
Profile Icon Profile Icon
Sign-in/Register UserName
Products

Link Arrow
Solutions

Link Arrow
Discover & Learn

Link Arrow
Resources & Tools

Link Arrow
Support

Link Arrow
Close Menu

The site is undergoing maintenance.  Ordering can continue through fax and phone.

If you encounter problems while ordering. Please contact us at BDBCustomerService@bd.com 

See More

Close
Website Feedback
Alert Icon
563607Image1.png

Flow cytometric analysis of mouse CD31 expression on mouse bone marrow cells. Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with PE Rat Anti-Mouse CD45R/B220 antibody (Cat. No. 553089/553090/561878) and either Alexa Fluor® 488 Rat IgG2a, κ Isotype Control (Cat. No. 557676, Left Panel) or Alexa Fluor® 488 Rat Anti-Mouse CD31 antibody (Cat. No. 563607, Right Panel). Two-color flow cytometric dot plots showing the correlated expression of CD31 (or Ig isotype control staining) and CD45R/B220 were derived from gated events with the light scattering characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometry System.

563607Image1.png

Flow cytometric analysis of mouse CD31 expression on mouse bone marrow cells. Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with PE Rat Anti-Mouse CD45R/B220 antibody (Cat. No. 553089/553090/561878) and either Alexa Fluor® 488 Rat IgG2a, κ Isotype Control (Cat. No. 557676, Left Panel) or Alexa Fluor® 488 Rat Anti-Mouse CD31 antibody (Cat. No. 563607, Right Panel). Two-color flow cytometric dot plots showing the correlated expression of CD31 (or Ig isotype control staining) and CD45R/B220 were derived from gated events with the light scattering characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometry System.

mCD31_A488_B220_PE.png

Flow cytometric analysis of mouse CD31 expression on mouse bone marrow cells. Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with PE Rat Anti-Mouse CD45R/B220 antibody (Cat. No. 553089/553090/561878) and either Alexa Fluor® 488 Rat IgG2a, κ Isotype Control (Cat. No. 557676, Left Panel) or Alexa Fluor® 488 Rat Anti-Mouse CD31 antibody (Cat. No. 563607, Right Panel). Two-color flow cytometric dot plots showing the correlated expression of CD31 (or Ig isotype control staining) and CD45R/B220 were derived from gated events with the light scattering characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometry System.

563607Image1.png 563607Image1.png mCD31_A488_B220_PE.png Green-Cap-Blue-Label.png

Flow cytometric analysis of mouse CD31 expression on mouse bone marrow cells. Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with PE Rat Anti-Mouse CD45R/B220 antibody (Cat. No. 553089/553090/561878) and either Alexa Fluor® 488 Rat IgG2a, κ Isotype Control (Cat. No. 557676, Left Panel) or Alexa Fluor® 488 Rat Anti-Mouse CD31 antibody (Cat. No. 563607, Right Panel). Two-color flow cytometric dot plots showing the correlated expression of CD31 (or Ig isotype control staining) and CD45R/B220 were derived from gated events with the light scattering characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometry System.

Flow cytometric analysis of mouse CD31 expression on mouse bone marrow cells. Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with PE Rat Anti-Mouse CD45R/B220 antibody (Cat. No. 553089/553090/561878) and either Alexa Fluor® 488 Rat IgG2a, κ Isotype Control (Cat. No. 557676, Left Panel) or Alexa Fluor® 488 Rat Anti-Mouse CD31 antibody (Cat. No. 563607, Right Panel). Two-color flow cytometric dot plots showing the correlated expression of CD31 (or Ig isotype control staining) and CD45R/B220 were derived from gated events with the light scattering characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometry System.

Flow cytometric analysis of mouse CD31 expression on mouse bone marrow cells. Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with PE Rat Anti-Mouse CD45R/B220 antibody (Cat. No. 553089/553090/561878) and either Alexa Fluor® 488 Rat IgG2a, κ Isotype Control (Cat. No. 557676, Left Panel) or Alexa Fluor® 488 Rat Anti-Mouse CD31 antibody (Cat. No. 563607, Right Panel). Two-color flow cytometric dot plots showing the correlated expression of CD31 (or Ig isotype control staining) and CD45R/B220 were derived from gated events with the light scattering characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometry System.

Product Details
Down Arrow Up Arrow


BD Pharmingen™
Platelet endothelial cell adhesion molecule; Pecam1; PECAM-1; Pecam-1
Mouse (QC Testing)
Rat LEW, also known as Lewis IgG2a, κ
C3H/HeJ mouse hematopoietic progenitor cell line 32D
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_2738312
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.
Show More blue down arrow Show Less blue up arrow

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  4. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
  5. Alexa Fluor® 488 fluorochrome emission is collected at the same instrument settings as for fluorescein isothiocyanate (FITC).
  6. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
  7. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Show More blue down arrow Show Less blue up arrow
563607 Rev. 1
Antibody Details
Down Arrow Up Arrow
390

The 390 monoclonal antibody specifically binds to CD31, also known as PECAM-1 (platelet endothelial cell adhesion molecule). CD31 is a ~130 kDa integral membrane glycoprotein, a member of the immunoglobulin superfamily, that mediates homophilic and heterophilic cell-cell adhesion. CD31 is expressed constitutively on the surface of adult and embryonic endothelial cells and is weakly expressed on many peripheral leukocytes and platelets. It has also been detected on bone marrow-derived hematopoietic stem cells and embryonic stem cells. CD31 is involved in the transendothelial emigration of neutrophils, and neutrophil PECAM-1 appears to be down-regulated after extravasation into inflamed tissues. Multiple alternatively spliced isoforms are detected during early post-implantation embryonic development; this alternative splicing is involved in regulation of ligand specificity. CD38 and vitronectin receptor (αvβ3 integrin, CD51/CD61) are proposed to be ligands for CD31. CD31-mediated endothelial cell-cell interactions are involved in angiogenesis. The 390 mAb inhibits a variety of in vitro and in vivo functions mediated by CD31.

563607 Rev. 1
Format Details
Down Arrow Up Arrow
Alexa Fluor™ 488
Alexa Fluor™ 488 Dye is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 517-nm. Alexa Fluor™ 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520-nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
Alexa Fluor™ 488
494 nm
517 nm
563607 Rev.1
Citations & References
Down Arrow Up Arrow
View product citations for antibody "563607" on CiteAb

Development References (13)

  1. Baldwin HS, Shen HM, Yan HC, et al. Platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31): alternatively spliced, functionally distinct isoforms expressed during mammalian cardiovascular development. Development. 1994; 120(9):2539-2953. (Immunogen: Bioassay, Immunoprecipitation). View Reference
  2. Buck CA, Baldwin HS, DeLisser H, et al. Cell adhesion receptors and early mammalian heart development: an overview. C R Acad Sci III. 1993; 316(9):849-859. (Biology). View Reference
  3. Buck CA, Edelman JM, Buck CE, Kennedy G, Baldwin HS. Expression patterns of adhesion receptors in the developing mouse lung: functional implications. Cell Adhes Commun. 1996; 4(2):69-87. (Biology). View Reference
  4. Christofidou-Solomidou M, Nakada MT, Williams J, Muller WA, DeLisser HM. Neutrophil platelet endothelial cell adhesion molecule-1 participates in neutrophil recruitment at inflammatory sites and is down-regulated after leukocyte extravasation. J Immunol. 1997; 158(10):4872-4878. (Biology). View Reference
  5. DeLisser HM, Christofidou-Solomidou M, Strieter RM, et al. Involvement of endothelial PECAM-1/CD31 in angiogenesis. Am J Pathol. 1997; 151(3):671-677. (Clone-specific: Blocking). View Reference
  6. DeLisser HM, Newman PJ, Albelda SM. Molecular and functional aspects of PECAM-1/CD31. Immunol Today. 1994; 15(10):490-495. (Biology). View Reference
  7. Duncan GS, Andrew DP, Takimoto H, et al. Genetic evidence for functional redundancy of Platelet/Endothelial cell adhesion molecule-1 (PECAM-1): CD31-deficient mice reveal PECAM-1-dependent and PECAM-1-independent functions. J Immunol. 1999; 162(5):3022-3030. (Biology). View Reference
  8. Famiglietti J, Sun J, DeLisser HM, Albelda SM. Tyrosine residue in exon 14 of the cytoplasmic domain of platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31) regulates ligand binding specificity. J Cell Biol. 1997; 138(6):1425-1435. (Biology). View Reference
  9. Horenstein AL, Stockinger H, Imhof BA, Malavasi F. CD38 binding to human myeloid cells is mediated by mouse and human CD31. Biochem J. 1998; 330(3):1129-1135. (Biology). View Reference
  10. Iguchi A, Okuyama R, Koguma M, Obinata M, Yanai N. Selective stimulation of granulopoiesis in vitro by established bone marrow stromal cells. Cell Struct Funct. 1997; 22(3):357-364. (Clone-specific: Blocking). View Reference
  11. Ling V, Luxenberg D, Wang J, et al. Structural identification of the hematopoietic progenitor antigen ER-MP12 as the vascular endothelial adhesion molecule PECAM-1 (CD31). Eur J Immunol. 1997; 27(2):509-514. (Biology). View Reference
  12. Piali L, Hammel P, Uherek C, et al. CD31/PECAM-1 is a ligand for alpha v beta 3 integrin involved in adhesion of leukocytes to endothelium. J Cell Biol. 1995; 130(2):451-460. (Biology). View Reference
  13. Rosenblum WI, Nelson GH, Wormley B, Werner P, Wang J, Shih CC. Role of platelet-endothelial cell adhesion molecule (PECAM) in platelet adhesion/aggregation over injured but not denuded endothelium in vivo and ex vivo. Stroke. 1996; 27(4):709-711. (Clone-specific: Blocking). View Reference
View All (13) View Less
563607 Rev. 1

Please refer to Support Documents for Quality Certificates

 

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

 

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.