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Alexa Fluor® 488 Mouse anti-Human HLA-ABC
Alexa Fluor® 488 Mouse anti-Human HLA-ABC

Flow cytometric analysis of Alexa 488 anti-human HLA-A,B,C on lymphocytes. Whole blood was stained with Alexa 488 anti-human HLA-A,B,C  (clone DX17 , Cat. No. 560169) and compared to whole blood stained with a Alexa 488 mouse IgG1 isotype control (clone MOPC-21, Cat. No. 557702).   The isotype control is represented by a dashed line and the Alexa 488 anti-human HLA-A,B,C by the solid line. Flow cytometry was performed on a BD FACSCalibur™ System and the histograms were derived from the gated events based on light scattering characteristics of viable cells.

Flow cytometric analysis of Alexa 488 anti-human HLA-A,B,C on lymphocytes. Whole blood was stained with Alexa 488 anti-human HLA-A,B,C  (clone DX17 , Cat. No. 560169) and compared to whole blood stained with a Alexa 488 mouse IgG1 isotype control (clone MOPC-21, Cat. No. 557702).   The isotype control is represented by a dashed line and the Alexa 488 anti-human HLA-A,B,C by the solid line. Flow cytometry was performed on a BD FACSCalibur™ System and the histograms were derived from the gated events based on light scattering characteristics of viable cells.

Product Details
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BD Pharmingen™
Human (QC Testing), Human (Reported)
Mouse IgG1, κ
Polyclonal Human NK Cell Line
Flow cytometry (Routinely Tested)
20 µl
AB_1645353
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  4. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
  5. Alexa Fluor® 488 fluorochrome emission is collected at the same instrument settings as for fluorescein isothiocyanate (FITC).
  6. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  7. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  8. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
560169 Rev. 1
Antibody Details
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DX17

The DX17 monoclonal antibody reacts with a monomorphic epitope expressed on all HLA (human leukocyte antigen) class I molecules examined.  DX17 immunoprecipitates HLA class I heavy chains (45 kDa) and β2-microglobulin (12kDa) from radiolabeled human cell lines.  HLA is determined by a complex segment of the short arm of chromosome 6 and there are many human HLAs encoded in this segment. The antigenic agglomerate is called MHC, for major histocompatibility complex. Examples of class I loci are HLA-A, -B, and -C, which are serologically assayed; class II loci, e.g., HLA-D/DR and DC1, are tested by lymphocytotoxic methods.

560169 Rev. 1
Format Details
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Alexa Fluor™ 488
Alexa Fluor™ 488 Dye is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 517-nm. Alexa Fluor™ 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520-nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 488
Blue 488 nm
494 nm
517 nm
560169 Rev.1
Citations & References
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Development References (4)

  1. Bach FH, Amos DB. Hu-1: Major histocompatibility locus in man. Science. 1967; 156(781):1506-1508. (Biology). View Reference
  2. Gumperz JE, Litwin V, Phillips JH, Lanier LL, Parham P. The Bw4 public epitope of HLA-B molecules confers reactivity with natural killer cell clones that express NKB1, a putative HLA receptor. J Exp Med. 1995; 181(3):1133-1144. (Biology). View Reference
  3. Lanier LL, Gumperz JE, Parham P, Melero I, López-Botet M, Phillips JH. The NKB1 and HP-3E4 NK cells receptors are structurally distinct glycoproteins and independently recognize polymorphic HLA-B and HLA-C molecules. J Immunol. 1995; 154(7):3320-3327. (Clone-specific). View Reference
  4. Morton CC, Kirsch IR, Nance WE, Evans GA, Korman AJ, Strominger JL. Orientation of loci within the human major histocompatibility complex by chromosomal in situ hybridization. Proc Natl Acad Sci U S A. 1984; 81(9):2816-2820. (Biology). View Reference
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560169 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.