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Alexa Fluor® 488 Mouse anti-GATA4
Alexa Fluor® 488 Mouse anti-GATA4

Flow cytometric analysis of GATA4 on mouse testis, embryonal carcinoma F9 cells.  F9 cells were either treated with retinoic acid/dbcAMP (solid line) or untreated (dashed line). The cells were fixed (BD Cytofix™ buffer Cat. No. 554655) for 10 minutes at 37ºC, then permeabilized with

BD Phosflow™ Perm Buffer III (Cat. No. 558050) on ice for 30 minutes. The cells were stained with Alexa Fluor® 488 Mouse Anti-GATA4 (Cat. No. 560330) and incubated in the dark for 30 minutes at room temperature. Flow cytometry was performed on a BD FACSCanto™ II flow cytometry system. This antibody also works in BD Phosflow™ Perm Buffer I and II.

Flow cytometric analysis of GATA4 on mouse testis, embryonal carcinoma F9 cells.  F9 cells were either treated with retinoic acid/dbcAMP (solid line) or untreated (dashed line). The cells were fixed (BD Cytofix™ buffer Cat. No. 554655) for 10 minutes at 37ºC, then permeabilized with

BD Phosflow™ Perm Buffer III (Cat. No. 558050) on ice for 30 minutes. The cells were stained with Alexa Fluor® 488 Mouse Anti-GATA4 (Cat. No. 560330) and incubated in the dark for 30 minutes at room temperature. Flow cytometry was performed on a BD FACSCanto™ II flow cytometry system. This antibody also works in BD Phosflow™ Perm Buffer I and II.

Product Details
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BD Pharmingen™
MGC126629, GATA-4
Mouse (QC Testing), Human (Reported)
Mouse BALB/c IgG1, κ
Human GATA4 Peptide
Intracellular staining (flow cytometry) (Routinely Tested)
42-50 kDa
20 µl
AB_1645182
Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Alexa Fluor® 488 fluorochrome emission is collected at the same instrument settings as for fluorescein isothiocyanate (FITC).
  5. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
  6. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
  7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  8. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
560330 Rev. 4
Antibody Details
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L97-56

The L97-56 monoclonal antibody reacts with GATA4 (GATA-binding protein 4), a member of the GATA family of zinc finger-containing transcription factors that bind to the GATA nucleotide sequence. This ~50 kDa nuclear protein is expressed in mesodermal and definitive endodermal tissues such as the gastrointestinal tract, gonads, and heart. Genetic studies suggest that GATA4 regulates embryonic cardiac development in mice, and disruption of the GATA4 gene leads to defects in heart tube formation; in humans, GATA4 mutations are associated with atrial septal defects. In the adult heart, GATA4 regulates differentiated gene expression. The roles of GATA4 in endocrine and reproductive functions were recently reviewed.

560330 Rev. 4
Format Details
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Alexa Fluor™ 488
Alexa Fluor™ 488 Dye is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 517-nm. Alexa Fluor™ 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520-nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 488
Blue 488 nm
494 nm
517 nm
560330 Rev.4
Citations & References
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Development References (2)

  1. Oka T, Xu J, Molkentin JD. Re-employment of developmental transcription factors in adult heart disease. Semin Cell Dev Biol. 2007; 18(1):117-131. (Biology). View Reference
  2. Viger RS, Guittot SM, Anttonen M, Wilson DB, Heikinheimo M. Role of the GATA family of transcription factors in endocrine development, function, and disease. Mol Endocrinol. 2008; 22(4):781-789. (Biology). View Reference
560330 Rev. 4

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.