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      Nile Red Fluorescent Particles, 2.5 - 4.5 µm
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      This SKU will be discontinuing Apr 2024. For additional support, contact your local applications specialist. Contact Us #

      Sphero™ Nile Red Fluorescent Particles, 2.5 - 4.5 µm

      (RUO)
      Nile Red Fluorescent Particles, 2.5 - 4.5 µm
      Excitation and emission spectra of SPHERO fluorescent particles in suspension with the exception with Nile Blue and Sky Blue, which were read from a solution of dye in styrene.
      Nile Red Fluorescent Particles, 2.5 - 4.5 µm
      Excitation and emission spectra of SPHERO fluorescent particles in suspension with the exception with Nile Blue and Sky Blue, which were read from a solution of dye in styrene.
      Product Details
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      Description

      The vial contains a mixture of 2.5 - 4.5 µm Nile Red Fluorescent Particles that are dyed to have an excitation maximum of approximately 525 nm with a subsequent emission maximum of approximately 560 nm. This particle mixture is useful with flow cytometers equipped with a 488 nm argon laser. This particle mixture has compatible excitation and emission spectra with R-Phycoerythrin and Propidium Iodide.



      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      Recommended Assay Procedures

      This particle mixture (~1.0% w/v) is useful for routine calibration of flow cytometers. Before use, resuspend the particles by vortexing. Dilution of 3 - 5 drops of particles to 1 ml of sheath fluid will provide an adequate number of particles for flow cytometric anaylsis.

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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      556269 Rev. 6

      Please refer to Support Documents for Quality Certificates

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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