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Purified Rat Anti-Mouse IgG2b
Product Details
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BD Pharmingen™
Mouse (QC Testing)
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1
Pooled mouse Ig
ELISA Capture (Routinely Tested), Flow cytometry (Reported)
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

For the sandwich mouse IgG2b ELISA, purified mAb R9-91 is optimal for capture with biotin anti-mouse IgG2b R12-3 mAb (Cat. No. 553393) for detection and Cat. No. 557351 as a standard. Purified R9-91 mAb may be used as a primary reagent in immunofluorescent staining. For flow cytometric detection of intracytoplasmic IgG2b, we recommend FITC-conjugated mAb R12-3 (Cat. No. 553395).

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
553396 Rev. 11
Antibody Details
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The R9-91 antibody recognizes an epitope in the CH3 domain of mouse IgG2b of Igh-C[a] and Igh-C[b] haplotypes. It does not react with other Ig isotypes. Detection of surface immunoglobulin on B lymphoma cells has been demonstrated with R9-91 mAb.

553396 Rev. 11
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
553396 Rev.11

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.