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Purified Rat Anti-Mouse CD74
Product Details
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BD Pharmingen™
Cd74; CLIP; Ii; Ia-associated invariant chain; Ia-GAMMA; DHLAG; HG2A; HLADG
Mouse (QC Testing)
Rat WF, also known as Wistar Furth IgG2b, κ
A.TL mouse lymphocytes and B10.A mouse-derived CH1 B-cell lymphoma
Intracellular staining (flow cytometry) (Routinely Tested), Immunohistochemistry-frozen, Immunohistochemistry-zinc-fixed (Tested During Development), Immunofluorescence, Immunoprecipitation, Western blot (Reported), Immunohistochemistry-formalin (antigen retrieval required) (Not Recommended)
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
555317 Rev. 6
Antibody Details
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The In-1 monoclonal antibody specifically binds to CD74, the MHC class II-associated Invariant chain (Ii), expressed in I-A+ cell populations. Although it is predominantly expressed intracellularly, it has also been detected on the cell surface of some B-cell lines and at low levels on a small number of B lymphocytes. In the mouse, alternatively spliced transcripts encode 31-kDa and 41-kDa isoforms, and the cell-surface CD74 has a chondroitin sulfate side chain which allows it to interact with CD44. CD74 is directly involved in the intracellular transport of MHC class II molecules and antigen presentation, and it affects the maturation of T and B lymphocytes.

555317 Rev. 6
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
555317 Rev.6
Citations & References
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View product citations for antibody "555317" on CiteAb

Development References (12)

  1. Bertolino P, Rabourdin-Combe C. The MHC class II-associated invariant chain: a molecule with multiple roles in MHC class II biosynthesis and antigen presentation to CD4+ T cells. Crit Rev Immunol. 1996; 16(4):359-379. (Biology). View Reference
  2. Bikoff EK, Huang LY, Episkopou V, van Meerwijk J, Germain RN, Robertson EJ. Defective major histocompatibility complex class II assembly, transport, peptide acquisition, and CD4+ T cell selection in mice lacking invariant chain expression. J Exp Med. 1993; 177(6):1699-1712. (Clone-specific: Immunoprecipitation). View Reference
  3. Bodmer H, Viville S, Benoist C, Mathis D. Diversity of endogenous epitopes bound to MHC class II molecules limited by invariant chain. Science. 1994; 263(5151):1284-1286. (Biology). View Reference
  4. Cresswell P. Assembly, transport, and function of MHC class II molecules. Annu Rev Immunol. 1994; 12:259-293. (Biology). View Reference
  5. Elliott EA, Drake JR, Amigorena S, et al. The invariant chain is required for intracellular transport and function of major histocompatibility complex class II molecules. J Exp Med. 1994; 179(2):681-694. (Clone-specific: Immunofluorescence). View Reference
  6. Farr A, DeRoos PC, Eastman S, Rudensky AY. Differential expression of CLIP:MHC class II and conventional endogenous peptide:MHC class II complexes by thymic epithelial cells and peripheral antigen-presenting cells. Eur J Immunol. 1996; 26(12):3185-3193. (Clone-specific: Immunohistochemistry, Western blot). View Reference
  7. Koch N, Koch S, Hämmerling GJ. Ia invariant chain detected on lymphocyte surfaces by monoclonal antibody. Nature. 1982; 299(5884):644-645. (Immunogen: Immunoprecipitation). View Reference
  8. Mehringer JH, Harris MR, Kindle CS, McCourt DW, Cullen SE. Characterization of fragments of the murine Ia-associated invariant chain. J Immunol. 1991; 146(3):920-927. (Clone-specific: Western blot). View Reference
  9. Nakagawa T, Roth W, Wong P, et al. Cathepsin L: critical role in Ii degradation and CD4 T cell selection in the thymus. Science. 1998; 280(5362):450-453. (Clone-specific: Immunohistochemistry, Western blot). View Reference
  10. Naujokas MF, Morin M, Anderson MS, Peterson M, Miller J. The chondroitin sulfate form of invariant chain can enhance stimulation of T cell responses through interaction with CD44. Cell. 1993; 74(2):257-268. (Clone-specific: Immunoprecipitation). View Reference
  11. Shachar I, Flavell RA. Requirement for invariant chain in B cell maturation and function. Science. 1996; 274(5284):106-108. (Biology). View Reference
  12. Viville S, Neefjes J, Lotteau V, et al. Mice lacking the MHC class II-associated invariant chain. Cell. 1993; 72(4):635-648. (Clone-specific: Immunohistochemistry). View Reference
View All (12) View Less
555317 Rev. 6

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.