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Purified Rat Anti-Mouse CD29
Product Details
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BD Pharmingen™
Integrin β1 chain
Mouse (QC Testing)
Rat IgG2a, κ
Mouse endothelial cell line
Flow cytometry (Routinely Tested), Immunohistochemistry-paraffin (Tested During Development), Functional assay, Immunoprecipitation, Western blot (Reported)
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

For IHC, we recommend the use of purified 9EG7 mAb in our special formulation for immunohistochemistry, Cat. No. 550531.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
553715 Rev. 14
Antibody Details
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The 9EG7 antibody reacts with the 130-kDa integrin β1 chain (CD29).  CD29 is expressed on the cell surface as a heterodimer with one of the distinct integrin α chains. With α1 through α6 (CD49a through CD49f), it forms the VLA-1 through VLA-6 complexes, respectively, and with αv (CD51), it forms αvβ1 integrin.  It  also associates with the integrin α7 α8 and α9 chains in non-lymphoid tissues.  As a result, CD29 has a broad tissue distribution, including lymphocytes, endothelia, smooth muscle, and epithelia.  9EG7 mAb has been shown to inhibit both the α6β1-mediated binding of lymphocytes to endothelial cells and the adhesion  mediated by activated, but not unactivated, α4β1-integrin.  The source of the immunogen was mouse lymph node-derived endothelial cell line TME.

553715 Rev. 14
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
553715 Rev.14
Citations & References
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Development References (9)

  1. Driessens MH, Van Rijthoven EA, Kemperman H, Roos E. Adhesion of lymphoma cells to fibronectin: differential use of alpha 4 beta 1 and alpha 5 beta 1 integrins and stimulation by the 9EG7 mAb against the murine beta 1 integrin subunit. Cell Adhes Commun. 1995; 3(4):327-336. (Clone-specific: Functional assay). View Reference
  2. Kassner PD, Kawaguchi S, Hemler ME. Minimum alpha chain cytoplasmic tail sequence needed to support integrin-mediated adhesion. J Biol Chem. 1994; 269(31):19859-19867. (Clone-specific: Functional assay). View Reference
  3. Lenter M, Uhlig H, Hamann A, Jenö P, Imhof B, Vestweber D. A monoclonal antibody against an activation epitope on mouse integrin chain beta 1 blocks adhesion of lymphocytes to the endothelial integrin alpha 6 beta 1. Proc Natl Acad Sci U S A. 1993; 90(19):9051-9055. (Immunogen: Functional assay, Immunoprecipitation). View Reference
  4. Lenter M, Vestweber D. The integrin chains beta 1 and alpha 6 associate with the chaperone calnexin prior to integrin assembly. J Biol Chem. 1994; 269(16):12263-12268. (Clone-specific: Functional assay, Immunoaffinity chromatography, Immunoprecipitation, Western blot). View Reference
  5. Milner R, Edwards G, Streuli C, Ffrench-Constant C. A role in migration for the alpha V beta 1 integrin expressed on oligodendrocyte precursors. J Neurosci. 1996; 16(22):7240-7252. (Clone-specific: Functional assay, Immunoprecipitation). View Reference
  6. Mouse Genome Database (MGD), Mouse Genome Informatics, The Jackson Laboratory. B2m, beta-2 microglobulin. Available: 4/15/1998.
  7. Rich S, Van Nood N, Lee HM. Role of alpha 5 beta 1 integrin in TGF-beta 1-costimulated CD8+ T cell growth and apoptosis. J Immunol. 1996; 157(7):2916-2923. (Clone-specific: Functional assay). View Reference
  8. Springer TA. Adhesion receptors of the immune system. Nature. 1990; 346(6283):425-434. (Clone-specific: Immunoprecipitation). View Reference
  9. Yashiro Y, Tai XG, Toyo-oka K, et al. A fundamental difference in the capacity to induce proliferation of naive T cells between CD28 and other co-stimulatory molecules. Eur J Immunol. 1998; 28(3):926-935. (Biology). View Reference
View All (9) View Less
553715 Rev. 14

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.