BD Pharmingen™ Purified Mouse Anti-Rat IgG2a

Purified Mouse Anti-Rat IgG2a (Cat. No. 553893) is routinely tested by ELISA for its ability to recognize and bind Rat IgG2a, kappa (clone R35-95) and Rat IgG2a, lambda (Clone B39-4) while not binding all other Rat Ig isotypes.  Biotin Rat Anti-Mouse IgG2b (clone R12-3; Cat. No. 553393) along with a streptavidin reporting system is used for detection.

For sandwich Rat IgG2a ELISA, Biotin Mouse Anti-Rat IgG2a (Cat. No. 553894) is optimal for detection with Purified Mouse Anti-Rat IgG2a (B46-7; Cat. No. 553918) for capture. RG7/1.30 antibody is effective for detection of cell-surface or intracellular Ig by immunofluorescent staining with flow cytometric analysis. For flow cytometric detection of intracytoplasmic IgG2a, we recommend FITC Mouse Anti-Rat IgG2a (Cat. No. 553896).

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.