BD Pharmingen™ Purified Mouse Anti-Human CD221
Clone 1H7 (RUO)
Preparation And Storage
Recommended Assay Procedures
Note: this product is routinely tested on peripheral blood granulocytes. The histogram is an example of the expected reactivity.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
The 1H7 monoclonal antibody specifically binds to the α subunit of the human insulin-like growth factor-I (IGF-I) receptor that is also known as CD221. CD221 is a receptor tyrosine kinase that has high affinity for IGF-1 and low affinity for insulin and IGF-2. CD221 is synthesized as a 180 kDa precursor molecule that is processed into two subunits, a 135 kDa α subunit which is disulfide-linked to a 90 kDa β subunit. The mature IGF-IR complex is comprised of two extracellular α subunits and two transmembrane β subunits that each possesses a tyrosine kinase domain. When bound by ligand, the IGF-1 receptor autophosphorylates multiple tyrosine sites in its β chains and phosphorylates other signaling molecules that regulate cell growth, development, and neoplastic transformation. CD221 is structurally and functionally similar to the insulin receptor (aka, CD220) and is expressed on a variety of human hematopoietic and non-hematopoietic cells. The 1H7 antibody does not crossreact with the human insulin receptor and can block IGF-I- or IGF-II-stimulated cell growth.
This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Development References (3)
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Kooijman RK, Scholtens LE, Rijkers GT, Zegers BJ. Differential expression of type I insulin-like growth factor receptors in different stages of human T cells. Eur J Immunol. 1995; 25(4):931-935. (Biology). View Reference
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Li SL, Kato J, Paz IB, Kasuya J, Fujita-Yamaguchi Y. Two new monoclonal antibodies against the alpha subunit of the human insulin-like growth factor-I receptor. Biochem Biophys Res Commun. 1993; 196(1):92-98. (Biology). View Reference
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Xiong L, Kasuya J, Li SL, Kato J, Fujita-Yamaguchi Y. Growth-stimulatory monoclonal antibodies against human insulin-like growth factor I receptor. Proc Natl Acad Sci U S A. 1992; 89(12):5356-5360. (Biology). View Reference
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.