Purified Mouse Anti-Human CD18

This SKU will be discontinuing Apr 2024. Suggested alternate SKU is [555922] or for additional support, contact your local applications specialist. Contact Us

BD Pharmingen™ Purified Mouse Anti-Human CD18

Name: Purified Mouse Anti-Human CD18
Brand: BD Pharmingen™
SKU: 556084

Clone L130

(RUO)

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Scientific Resources

Profile of peripheral blood lymphocytes analyzed on a FACScan (BDIS, San Jose, CA)

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Scientific Resources

Product Details

Brand: BD Pharmingen™

Reactivity: Human (QC Testing)

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Thymic TCR γδ+ T lymphocytes

Application: Flow cytometry (Routinely Tested)

Concentration: 0.5 mg/ml

Workshop Number: V S166

Entrez Gene ID: 3689

RRID: AB_396349

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Companion Products

Purified Mouse IgG1, κ Isotype Control RUO

Size 0.1 mg Cat No. 555746

FITC Goat Anti-Mouse IgG/IgM RUO

Size 0.5 mg Cat No. 555988

556084 Rev. 24

Antibody Details

L130

The L130 monoclonal antibody specifically binds to CD18 which is also known as β2 integrin. CD18 is an ~90-95 kDa type I transmembrane glycoprotein that is encoded by ITGB2. CD18 associates with the integrin α chains CD11a, CD11b, CD11c, or CD11d to form the LFA-1, Mac-1, p150,95, or αDβ2 heterodimers, respectively. CD18 is expressed on virtually all normal peripheral blood leucocytes. CD18 serves as a leucocyte adhesion receptor and signaling receptor. It is essential for many immune responses that require cell-to-cell contact, such as lymphocyte adhesion, natural killer (NK) cell and T-lymphocyte cytolysis, and T lymphocyte proliferation.

556084 Rev. 24

Format Details

Purified

Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.

Format: Purified

556084 Rev.24

Citations & References

Development References (3)

Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997.
Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
Springer TA. Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm. Cell. 1994; 76(2):301-314. (Biology). View Reference

556084 Rev. 24

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.