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BD Pharmingen™ PE Rat Anti-Mouse Zbtb20
Clone 4A3 (RUO)
Multicolor flow cytometric analysis of Zbtb20 expression in B1 or B2 subsets of mouse peritoneal B cells. Peritoneal cells were stained with APC-Cy™7 Rat Anti-Mouse CD45R/B220 (Cat. No. 552094), BD Horizon™ BV421 Rat Anti-Mouse IgM (Cat. No. 562595 ), and BD Horizon™ PE-CF594 Rat Anti-Mouse CD23 (Cat. No. 563986) antibodies. The cells were then washed, fixed, and permeabilized using the BD Pharmingen™ Transcription Factor Buffer Set (Cat. No. 562574/562725) and stained with either PE Rat IgG2a, κ Isotype Control (Cat. No. 554689; dashed line histograms) or PE Rat Anti-Mouse Zbtb20 antibody (Cat. No. 565454; solid line histograms). The fluorescence histograms showing Zbtb20 expression (or Ig Isotype control staining) were derived from either B220+ IgM+ CD23- (B1 cells; Top Right Plot) or B220+ IgM low CD23+ (B2 cells; Bottom Right Plot) gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Multicolor flow cytometric analysis of Zbtb20 expression in B1 or B2 subsets of mouse peritoneal B cells. Peritoneal cells were stained with APC-Cy™7 Rat Anti-Mouse CD45R/B220 (Cat. No. 552094), BD Horizon™ BV421 Rat Anti-Mouse IgM (Cat. No. 562595 ), and BD Horizon™ PE-CF594 Rat Anti-Mouse CD23 (Cat. No. 563986) antibodies. The cells were then washed, fixed, and permeabilized using the BD Pharmingen™ Transcription Factor Buffer Set (Cat. No. 562574/562725) and stained with either PE Rat IgG2a, κ Isotype Control (Cat. No. 554689; dashed line histograms) or PE Rat Anti-Mouse Zbtb20 antibody (Cat. No. 565454; solid line histograms). The fluorescence histograms showing Zbtb20 expression (or Ig Isotype control staining) were derived from either B220+ IgM+ CD23- (B1 cells; Top Right Plot) or B220+ IgM low CD23+ (B2 cells; Bottom Right Plot) gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Product Details
BD Pharmingen™
DPZF; HOF; ODA-8S; Oda8; Zfp288
Mouse (QC Testing)
Rat IgG2a, κ
Mouse Zbtb20 Peptide
Intracellular staining (flow cytometry) (Routinely Tested)
0.2 mg/ml
AB_2739245
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Cy is a trademark of GE Healthcare.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 ML
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 ML
Cat No.
554657
PE Rat IgG2a, κ Isotype Control RUO
Size
0.1 mg
Cat No.
554689
APC-Cy™7 Rat Anti-Mouse CD45R RUO
Size
0.1 mg
Cat No.
552094
PE-CF594 Rat Anti-Mouse CD23 RUO
Size
50 µg
Cat No.
563986
BV421 Rat Anti-Mouse IgM RUO
Size
50 µg
Cat No.
562595
565454 Rev. 2
Antibody Details
4A3
The 4A3 monoclonal antibody specifically binds to Zinc finger and BTB domain containing 20 (Zbtb20). This transcription factor is also known as Zinc finger protein 288 (Zfp288), BTB/POZ domain zinc finger factor HOF, BTB/POZ zinc finger protein DPZF, or POZ/zinc finger transcription factor ODA-8. Zbtb20 is a transcription factor that localizes to the nucleus and contains one BTB/POZ domain and 5 C2H2-type zinc fingers. Mouse ZBTB20 is expressed in thymus, spleen, lymph node, peritoneal cells and fetal liver. It is highly expressed in B1 and germinal center B cells, and long-lived antibody-secreting plasma cells. In cell lines, Zbtb20 is expressed at low levels in the immature WEHI231 and the mature 129B cell lines but at high levels in A20 cells, a germinal center B cell lymphoma. This transcription factor reportedly plays key roles in hematopoiesis, immunity, and oncogenesis.
565454 Rev. 2
Format Details
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
496 nm, 566 nm
576 nm
565454 Rev.2
Citations & References
View product citations for antibody "565454" on CiteAb
Development References (2)
-
Chevrier S, Emslie D, Shi W, et al. The BTB-ZF transcription factor Zbtb20 is driven by Irf4 to promote plasma cell differentiation and longevity. J Exp Med. 2014; 211(5):827-840. (Immunogen: Flow cytometry, Western blot). View Reference
-
Xie Z, Ma X, Ji W, et al. Zbtb20 is essential for the specification of CA1 field identity in the developing hippocampus. Proc Natl Acad Sci U S A. 2010; 107(14):6510-6515. (Biology). View Reference
565454 Rev. 2
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.