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PE Mouse Anti-Mouse I-A[b]
Product Details
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BD Pharmingen™
Mouse (QC Testing)
Mouse BALB/c IgG2a, κ
C57BL/10J Mouse Splenocytes
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_394919
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
553552 Rev. 13
Antibody Details
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AF6-120.1

The AF6-120.1 antibody reognizes the I-A[b] MHC class II alloantigen. It crossreacts with cells from mice of the H-2[k] and H-2[u] haplotypes. Reactivity with other haplotypes (e.g., d, f, g7, p, q, r, s) has not been observed.

553552 Rev. 13
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 561 nm
496 nm, 566 nm
576 nm
553552 Rev.13
Citations & References
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View product citations for antibody "553552" on CiteAb

Development References (5)

  1. Beck BN, Buerstedde JM, Krco CJ, Nilson AE, Chase CG, McKean DJ. Characterization of cell lines expressing mutant I-Ab and I-Ak molecules allows the definition of distinct serologic epitopes on A alpha and A beta polypeptides. J Immunol. 1986; 136(8):2953-2961. (Clone-specific: Flow cytometry). View Reference
  2. Cohn LE, Glimcher LH, Waldmann RA, et al. Identification of functional regions on the I-Ab molecule by site-directed mutagenesis. Proc Natl Acad Sci U S A. 1986; 83(3):747-751. (Clone-specific: Flow cytometry). View Reference
  3. Hattori M, Buse JB, Jackson RA, et al. The NOD mouse: recessive diabetogenic gene in the major histocompatibility complex. Science. 1986; 231(4739):733-735. (Biology). View Reference
  4. Nabozny GH, Baisch JM, Cheng S, et al. HLA-DQ8 transgenic mice are highly susceptible to collagen-induced arthritis: a novel model for human polyarthritis. J Exp Med. 1996; 183(1):27-37. (Clone-specific: Flow cytometry). View Reference
  5. Wall KA, Lorber MI, Loken MR, McClatchey S, Fitch FW. Inhibition of proliferation of MIs- and Ia-reactive cloned T cells by a monoclonal antibody against a determinant shared by I-A and I-E.. J Immunol. 1983; 131(3):1056-64. (Clone-specific: Flow cytometry). View Reference
View All (5) View Less
553552 Rev. 13

 

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.