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BD Pharmingen™ FITC Mouse Anti-Human HLA-DR, DP, DQ
Clone Tu39 (also known as TÜ39) (RUO)
Flow cytometric analysis of HLA-DR, DP, DQ expression on Rhesus macaque (Macaca mulatta) peripheral blood granulocytes. Rhesus whole blood was stained with either FITC Mouse Anti-Human HLA-DR, DP, DQ (Cat. No. 550853/555558/562008; solid line histogram) or FITC Mouse IgG2a, κ Isotype Control (Cat. No. 556652; dashed line histogram). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable granulocytes.
Flow cytometric analysis of HLA-DR, DP, DQ expression on Rhesus macaque (Macaca mulatta) peripheral blood granulocytes. Rhesus whole blood was stained with either FITC Mouse Anti-Human HLA-DR, DP, DQ (Cat. No. 550853/555558/562008; solid line histogram) or FITC Mouse IgG2a, κ Isotype Control (Cat. No. 556652; dashed line histogram). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable granulocytes.
Product Details
BD Pharmingen™
Tü39; MHC class II HLA-DR, DP, DQ
Rhesus, Cynomolgus, Baboon (QC Testing), Human (Tested in Development)
Mouse IgG2a, κ
Flow cytometry (Routinely Tested)
20 µl
AB_393926
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 ML
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 ML
Cat No.
554657
Lysing Buffer RUO
Size
100 ML
Cat No.
555899
FITC Mouse IgG2a, κ Isotype Control RUO
Size
50 Tests
Cat No.
556652
FITC Mouse Anti-Human HLA-DR, DP, DQ RUO
Size
25 Tests
Cat No.
562008
FITC Mouse Anti-Human HLA-DR, DP, DQ RUO
Size
100 Tests
Cat No.
555558
550853 Rev. 2
Antibody Details
Tu39
The TU39 monoclonal antibody specifically recognizes human major histocompatibility (MHC) Class II HLA-DR, DP and most DQ antigens. These antigens are encoded by genes within the Human Leukocyte Antigen (HLA) Complex located on chromosome 6. MHC Class II antigens are transmembrane heterodimeric glycoproteins composed of α chain (36 kDa) and β chain (27 kDa) subunits. They are expressed primarily on antigen presenting cells which include dendritic cells, monocytes, macrophages, thymic epithelial cells, and B cells. They are also expressed on activated T cells. This molecule plays a major role in mediating cellular interactions during antigen presentation to CD4+ T lineage cells. The TU39 antibody is reportedly useful for immunophenotyping as well as functional studies including the inhibition of mixed lymphocyte reactions and antibody-mediated complement fixation on target cells.
550853 Rev. 2
Format Details
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
FITC
494 nm
518 nm
550853 Rev.2
Citations & References
View product citations for antibody "550853" on CiteAb
Development References (1)
-
Barclay NA, Brown MH, Birkeland ML, et al, ed. The Leukocyte Antigen FactsBook. San Diego, CA: Academic Press; 1997.
550853 Rev. 2
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.