The ALULA monoclonal antibody specifically binds to Integrin alpha v beta 5 (αvβ5), which is also known as CD51/β5. Integrin αvβ5 is widely expressed by fibroblasts, endothelial cells, and vascular smooth muscle cells. It is involved in activation-dependent cell migration and cell adhesion to the extracellular matrix. Integrin αvβ5 plays a major role in growth factor-induced angiogenesis in response to VEGF and TGF. The αv Integrins, αvβ5 and αvβ3, αre involved in tumor growth and are being targeted in cancer therapies. The ALULA clone specifically recognizes αvβ5 integrin and functionally blocks the interaction between αvβ5 and vitronectin. Although raised against mouse αvβ5, the ALULA antibody crossreacts with human αvβ5.
The antibody was conjugated to BD Horizon™ BUV496 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348-nm and an acceptor dye with an Em Max at 496-nm. BD Horizon BUV496 can be excited by the ultraviolet laser (355 nm) and detected with a 515/30 nm filter with a 450LP. Due to the excitation of the acceptor dye by other laser lines, there may be significant spillover into the channel detecting BD Horizon V500 or BV510 (eg, 525/40-nm filter). However, the spillover can be corrected through compensation as with any other dye combination.