Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
For optimal and reproducible results, BD Horizon Brilliant™ Stain Buffer should be used anytime BD Horizon Brilliant™ dyes are used in a multicolor flow cytometry panel. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).
For optimal results, it is recommended to perform 2 washes after staining with antibodies. Cells may be prepared, stained with antibodies and washed twice with wash buffer per established protocols for immunofluorescence staining, prior to acquisition on a flow cytometer. Performing fewer than the recommended wash steps may lead to increased spread of the negative population.
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
- Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Alexa Fluor™ is a trademark of Life Technologies Corporation.
- For U.S. patents that may apply, see bd.com/patents.
Companion Products
The 3C12 monoclonal antibody specifically recognizes Integrin alpha 7 (Integrin α7 or α7 Integrin). Integrins mediate important intercellular and cell-extracellular matrix interactions in a variety of biological processes. Integrin α7 is a single-pass type I transmembrane glycoprotein that is encoded by ITGA7 (Integrin subunit alpha 7) which belongs to the Integrin alpha chain family. Integrin α7 is mainly expressed by skeletal, cardiac, and smooth muscle cells. It associates with Integrin β1 to form a heterodimeric Integrin α7β1 complex. This heterodimer functions as the major laminin-binding receptor. Integrin α7β1 mediates cellular binding to components of the extracellular matrix including laminin-1 as well as the laminin isoforms-2 and -4. Integrin α7β1 participates in the adhesion and motility of myoblasts during myogenesis and helps guide muscle fiber formation and maintenance.
Development References (10)
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Burkin DJ, Kaufman SJ. The alpha7beta1 integrin in muscle development and disease.. Cell Tissue Res. 1999; 296(1):183-90. (Biology). View Reference
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Kobayashi N, Oda T, Takizawa M, et al. Integrin α7 and Extracellular Matrix Laminin 211 Interaction Promotes Proliferation of Acute Myeloid Leukemia Cells and Is Associated with Granulocytic Sarcoma.. Cancers (Basel). 2020; 12(2):E363. (Biology). View Reference
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Liu J, Burkin DJ, Kaufman SJ. Increasing alpha 7 beta 1-integrin promotes muscle cell proliferation, adhesion, and resistance to apoptosis without changing gene expression.. Am J Physiol Cell Physiol. 2008; 294(2):C627-40. (Biology). View Reference
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Mayer U, Saher G, Fässler R, et al. Absence of integrin alpha 7 causes a novel form of muscular dystrophy.. Nat Genet. 1997; 17(3):318-23. (Biology). View Reference
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Mielenz D, Hapke S, Pöschl E, von Der Mark H, von Der Mark K. The integrin alpha 7 cytoplasmic domain regulates cell migration, lamellipodia formation, and p130CAS/Crk coupling.. J Biol Chem. 2001; 276(16):13417-26. (Biology: Flow cytometry). View Reference
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Rosbottom A, Scudamore CL, von der Mark H, Thornton EM, Wright SH, Miller HR. TGF-beta 1 regulates adhesion of mucosal mast cell homologues to laminin-1 through expression of integrin alpha 7.. J Immunol. 2002; 169(10):5689-95. (Clone-specific: Flow cytometry, Functional assay). View Reference
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Schöber S, Mielenz D, Echtermeyer F, et al. The role of extracellular and cytoplasmic splice domains of alpha7-integrin in cell adhesion and migration on laminins.. Exp Cell Res. 2000; 255(2):303-13. (Immunogen). View Reference
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Su Y, Guan XQ, Liu FQ, Wang YL. The effects of MIBG on the invasive properties of HepG2 hepatocellular carcinoma cells.. Int J Mol Med. 2014; 34(3):842-8. (Biology). View Reference
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Ziober BL, Vu MP, Waleh N, Crawford J, Lin CS, Kramer RH. Alternative extracellular and cytoplasmic domains of the integrin alpha 7 subunit are differentially expressed during development.. J Biol Chem. 1993; 268(35):26773-83. (Biology). View Reference
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von der Mark H, Williams I, Wendler O, Sorokin L, von der Mark K, Pöschl E. Alternative splice variants of alpha 7 beta 1 integrin selectively recognize different laminin isoforms.. J Biol Chem. 2002; 277(8):6012-6. (Clone-specific: Functional assay). View Reference
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