Flow cytometric analysis of CD182 expression on human peripheral blood granulocytes. Whole blood was stained with either APC Mouse IgG1, κ Isotype Control (Cat. No. 555751; dashed line histogram) or APC Mouse Anti-Human CD182 (Cat. No. 551127; solid line histogram). Erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). Fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable granulocytes.
Flow cytometric analysis of CD182 expression on human peripheral blood granulocytes. Whole blood was stained with either APC Mouse IgG1, κ Isotype Control (Cat. No. 555751; dashed line histogram) or APC Mouse Anti-Human CD182 (Cat. No. 551127; solid line histogram). Erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). Fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable granulocytes.
Product Details
BD Pharmingen™
CXCR2; CXCR-2; IL8RB; IL-8Rβ; Interleukin 8 receptor, beta; CDw128b
Human (QC Testing)
Mouse IgG1, λ
Human CXCR2 Transfected Cell Line
Flow cytometry (Routinely Tested)
20 µl
3579
AB_398492
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
Lysing Solution 10X Concentrate IVD
Size
100 mL
Cat No.
349202
Lysing Buffer RUO
Size
100 mL
Cat No.
555899
APC Mouse IgG1, κ Isotype Control RUO
Size
100 Tests
Cat No.
555751
551127 Rev. 3
Antibody Details
6C6
The 6C6 monoclonal antibody specifically binds to CD182. CD182 is also known as the interleukin-8 receptor B (IL-8RB), a G-protein-linked seven transmembrane domain protein, expressed on monocytes and granulocytes. It is also expressed on a subset of T cells. Both IL-8RA (CXCR1) and IL-8RB (CXCR2) are high affinity receptors for IL-8. IL-8 is an inflammatory cytokine which induces chemotaxis of neutrophils, basophils and T lymphocytes.
551127 Rev. 3
Format Details
APC
Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
APC
Red 627-640 nm
651 nm
660 nm
551127 Rev.3
Citations & References
View product citations for antibody "551127" on CiteAb
Development References (5)
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Baggiolini M, Loetscher P, Moser B. Interleukin-8 and the chemokine family. Int J Immunopharmacol. 1995; 17(2):103-108. (Biology). View Reference
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Barclay NA, Brown MH, Birkeland ML, et al, ed. The Leukocyte Antigen FactsBook. San Diego, CA: Academic Press; 1997.
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Callard R, Gearing A. Callard R, Gearing A. The Cytokine Facts Book. San Diego: Academic Press; 1994.
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Qin S, LaRosa G, Campbell JJ, et al. Expression of monocyte chemoattractant protein-1 and interleukin-8 receptors on subsets of T cells: correlation with transendothelial chemotactic potential. Eur J Immunol. 1996; 26(3):640-647. (Biology). View Reference
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Wu L, Ruffing N, Shi X, et al. Discrete steps in binding and signaling of interleukin-8 with its receptor.. J Biol Chem. 1996; 271(49):31202-9. (Biology). View Reference
551127 Rev. 3
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.