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561136Image1.png

Flow cytometric analysis of CD45RO expression on human peripheral blood lymphocytes.

     Left Panel: Whole blood was stained with Alexa Fluor® 700 Mouse Anti-Human CD45RO antibody (Cat. No. 561136; solid line histogram), or with a Alexa Fluor® 700 Mouse IgG2a, κ Isotype Control (Cat. No. 557880; dashed  line histogram).  

     Right Panel: Whole blood was stained with Alexa Fluor® 700 Mouse Anti-Human CD45RO antibody (Cat. No. 561136) in conjunction with a PE Mouse Anti-Human CD45RA antibody (Cat. No. 555489).

     Erythrocytes were lysed with BD PharmLyse™ Lysing Buffer (Cat. No.555899). The fluorescence histograms and dot plot were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

561136Image1.png

Flow cytometric analysis of CD45RO expression on human peripheral blood lymphocytes.

     Left Panel: Whole blood was stained with Alexa Fluor® 700 Mouse Anti-Human CD45RO antibody (Cat. No. 561136; solid line histogram), or with a Alexa Fluor® 700 Mouse IgG2a, κ Isotype Control (Cat. No. 557880; dashed  line histogram).  

     Right Panel: Whole blood was stained with Alexa Fluor® 700 Mouse Anti-Human CD45RO antibody (Cat. No. 561136) in conjunction with a PE Mouse Anti-Human CD45RA antibody (Cat. No. 555489).

     Erythrocytes were lysed with BD PharmLyse™ Lysing Buffer (Cat. No.555899). The fluorescence histograms and dot plot were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

CD45RA_CD45RO_A700.png

Flow cytometric analysis of CD45RO expression on human peripheral blood lymphocytes.

     Left Panel: Whole blood was stained with Alexa Fluor® 700 Mouse Anti-Human CD45RO antibody (Cat. No. 561136; solid line histogram), or with a Alexa Fluor® 700 Mouse IgG2a, κ Isotype Control (Cat. No. 557880; dashed  line histogram).  

     Right Panel: Whole blood was stained with Alexa Fluor® 700 Mouse Anti-Human CD45RO antibody (Cat. No. 561136) in conjunction with a PE Mouse Anti-Human CD45RA antibody (Cat. No. 555489).

     Erythrocytes were lysed with BD PharmLyse™ Lysing Buffer (Cat. No.555899). The fluorescence histograms and dot plot were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

561136Image1.png 561136Image1.png CD45RA_CD45RO_A700.png Blue-Cap-Red-Label.jpg

Flow cytometric analysis of CD45RO expression on human peripheral blood lymphocytes.

     Left Panel: Whole blood was stained with Alexa Fluor® 700 Mouse Anti-Human CD45RO antibody (Cat. No. 561136; solid line histogram), or with a Alexa Fluor® 700 Mouse IgG2a, κ Isotype Control (Cat. No. 557880; dashed  line histogram).  

     Right Panel: Whole blood was stained with Alexa Fluor® 700 Mouse Anti-Human CD45RO antibody (Cat. No. 561136) in conjunction with a PE Mouse Anti-Human CD45RA antibody (Cat. No. 555489).

     Erythrocytes were lysed with BD PharmLyse™ Lysing Buffer (Cat. No.555899). The fluorescence histograms and dot plot were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

Flow cytometric analysis of CD45RO expression on human peripheral blood lymphocytes.

     Left Panel: Whole blood was stained with Alexa Fluor® 700 Mouse Anti-Human CD45RO antibody (Cat. No. 561136; solid line histogram), or with a Alexa Fluor® 700 Mouse IgG2a, κ Isotype Control (Cat. No. 557880; dashed  line histogram).  

     Right Panel: Whole blood was stained with Alexa Fluor® 700 Mouse Anti-Human CD45RO antibody (Cat. No. 561136) in conjunction with a PE Mouse Anti-Human CD45RA antibody (Cat. No. 555489).

     Erythrocytes were lysed with BD PharmLyse™ Lysing Buffer (Cat. No.555899). The fluorescence histograms and dot plot were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

Flow cytometric analysis of CD45RO expression on human peripheral blood lymphocytes.

     Left Panel: Whole blood was stained with Alexa Fluor® 700 Mouse Anti-Human CD45RO antibody (Cat. No. 561136; solid line histogram), or with a Alexa Fluor® 700 Mouse IgG2a, κ Isotype Control (Cat. No. 557880; dashed  line histogram).  

     Right Panel: Whole blood was stained with Alexa Fluor® 700 Mouse Anti-Human CD45RO antibody (Cat. No. 561136) in conjunction with a PE Mouse Anti-Human CD45RA antibody (Cat. No. 555489).

     Erythrocytes were lysed with BD PharmLyse™ Lysing Buffer (Cat. No.555899). The fluorescence histograms and dot plot were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

Product Details
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BD Pharmingen™
CD45R; PTPRC; LCA; Leukocyte common antigen; GP180; LY5; T200
Human (QC Testing)
Mouse BALB/c IgG2a, κ
Human IL-2-dependent T-cell line
Flow cytometry (Routinely Tested)
5 µl
IV N31
AB_10565971
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 700 under optimum conditions, and unreacted Alexa Fluor® 700 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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Product Notices

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. Alexa Fluor® 700 has an adsorption maximum of ~700nm and a peak fluorescence emission of ~720nm. Before staining cells with this reagent, please confirm that your flow cytometer is capable of exciting the fluorochrome and discriminating the resulting fluorescence.
  3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  4. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
  7. An isotype control should be used at the same concentration as the antibody of interest.
  8. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
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561136 Rev. 1
Antibody Details
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UCHL1

The UCHL1 monoclonal antibody specifically binds to the 180 kDa isoform of CD45 (aka, the Leukocyte Common Antigen). CD45RO is a type I transmembrane glycoprotein that has cytoplasmic protein tyrosine phosphatase activity and functions in signal transduction pathways. This CD45 isoform does not include amino acid sequences encoded by the variable CD45 exons A, B, or C. CD45RO is expressed on most thymocytes, activated T cells, memory T cells, granulocytes and monocytes, but only on a proportion of resting T cells. CD45RO and CD45RA antibodies seem to define complementary, predominantly non-overlapping, populations in resting peripheral T cells, demonstrating heterogeneity within the CD8 and CD4 subpopulations. CD45RO binds to CD22.

561136 Rev. 1
Format Details
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Alexa Fluor™ 700
Alexa Fluor™ 700 dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 697 nm and an emission maximum (Em Max) at 719-nm. Alexa Fluor™ 700 is designed to be excited by the Red (627–640-nm) laser and detected using an optical filter centered near 720-nm (e.g., a 720/40-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
Alexa Fluor™ 700
697 nm
719 nm
561136 Rev.1
Citations & References
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View product citations for antibody "561136" on CiteAb

Development References (9)

  1. Barclay NA, Brown MH, Birkeland ML, et al, ed. The Leukocyte Antigen FactsBook. San Diego, CA: Academic Press; 1997.
  2. Johnson P, Maiti A, Ng DHW. CD45: A family of leukocyte-specific cell surface glycoproteins. In: Herzenberg LA, Weir DM, Herzenberg LA, Blackwell C , ed. Weir's Handbook of Experimental Immunology, Vol 2. Cambridge: Blackwell Science; 1997:62.1-62.16.
  3. Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
  4. Norton AJ, Ramsay AD, Smith SH, Beverley PC, Isaacson PG. Monoclonal antibody (UCHL1) that recognises normal and neoplastic T cells in routinely fixed tissues. J Clin Pathol. 1986; 39(4):399-405. (Immunogen: Immunohistochemistry). View Reference
  5. Picker LJ, Treer JR, Ferguson-Darnell B, Collins PA, Buck D, Terstappen LW. Control of lymphocyte recirculation in man. I. Differential regulation of the peripheral lymph node homing receptor L-selectin on T cells during the virgin to memory cell transition. J Immunol. 1993; 150(3):1105-1121. (Biology). View Reference
  6. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
  7. Schwinzer R. Cluster Report: CD45/CD45R. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:628-634.
  8. Smith SH, Brown MH, Rowe D, Callard RE, Beverley PC. Functional subsets of human helper-inducer cells defined by a new monoclonal antibody, UCHL1. Immunology. 1986; 58(1):63-70. (Biology). View Reference
  9. Streuli M, Morimoto C, Schrieber M, Schlossman SF, Saito H. Characterization of CD45 and CD45R monoclonal antibodies using transfected mouse cell lines that express individual human leukocyte common antigens. J Immunol. 1988; 141(11):3910-3914. (Biology). View Reference
View All (9) View Less
561136 Rev. 1

Please refer to Support Documents for Quality Certificates

 

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

 

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.