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Flow cytometric analysis of Integrin αVβ5 expression on human and mouse cell lines. Cells from the SW480 (Human colon adenocarcinoma, ATCC CCL-228; Left Plot), HT-1080 (Human fibrosarcoma, ATCC CCL-121; Middle Plot), or NIH/3T3 (Mouse embryonic fibroblast, ATCC CRL-1658; Right Plot) cell lines were stained with either Alexa Fluor® 647 Mouse IgG2b, κ Isotype Control (Cat. No. 565378; dashed line histograms) or Alexa Fluor® 647 Mouse Anti-Integrin αvβ5 antibody (Cat. No. 565836; solid line histograms). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a FACSCanto™ II Flow Cytometer System. Please note that the cells were maintained at 4°C throughout staining and analysis for optimal results.


BD Pharmingen™ Alexa Fluor® 647 Mouse Anti-Integrin αvβ5

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Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Recommended Assay Procedures
Investigators: Please note that for optimal results, the target cells should be maintained at 4°C throughout staining and analysis .
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- An isotype control should be used at the same concentration as the antibody of interest.
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The ALULA monoclonal antibody specifically binds to Integrin alpha v beta 5 (αvβ5), which is also known as CD51/β5. Integrin αvβ5 is widely expressed by fibroblasts, endothelial cells, and vascular smooth muscle cells. It is involved in activation-dependent cell migration and cell adhesion to the extracellular matrix. Integrin αvβ5 plays a major role in growth factor-induced angiogenesis in response to VEGF and TGF. The αv Integrins, αvβ5 and αvβ3, αre involved in tumor growth and are being targeted in cancer therapies. The ALULA clone specifically recognizes αvβ5 integrin and functionally blocks the interaction between αvβ5 and vitronectin. Although raised against mouse αvβ5, the ALULA antibody crossreacts with human αvβ5.
Development References (3)
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Burvenich I, Schoonooghe S, Vervoort L, et al. Monoclonal antibody 14C5 targets integrin alphavbeta5.. Mol Cancer Ther. 2008; 7(12):3771-9. (Biology). View Reference
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Lee P, Bax DV, Bilek MM, Weiss AS. A novel cell adhesion region in tropoelastin mediates attachment to integrin αVβ5.. J Biol Chem. 2014; 289(3):1467-77. (Biology). View Reference
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Su G1, Hodnett M, Wu N, et al. Am J Respir Cell Mol Biol. 2007; 36(3):377-386. (Immunogen: Blocking, Flow cytometry, Immunoprecipitation, Inhibition). View Reference
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.