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      Alexa Fluor® 647 Mouse anti-IGF-1 Receptor (CD221) (pY1131)
      Alexa Fluor® 647 Mouse anti-IGF-1 Receptor (CD221) (pY1131)
      Analysis of IGF-1 Receptor (pY1131) in transformed human epithelial cells.  Serum-starved 293 fetal kidney cells were either stimulated at 37°C for 2 minutes with 100 nM  IGF-I (shaded histogram) or unstimulated (open histogram).  The cells were fixed with pre-warmed BD Cytofix™ buffer (Cat. No. 554655) for 10 minutes at 37°C, then permeabilized (BD Phosflow™ Perm Buffer III, Cat. No. 558050) on ice for at least 30 minutes, and then stained with Alexa Fluor® 647 Mouse anti-IGF-1 Receptor (CD221) (pY1131, Cat. No. 558588).  Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
      Alexa Fluor® 647 Mouse anti-IGF-1 Receptor (CD221) (pY1131)
      Analysis of IGF-1 Receptor (pY1131) in transformed human epithelial cells.  Serum-starved 293 fetal kidney cells were either stimulated at 37°C for 2 minutes with 100 nM  IGF-I (shaded histogram) or unstimulated (open histogram).  The cells were fixed with pre-warmed BD Cytofix™ buffer (Cat. No. 554655) for 10 minutes at 37°C, then permeabilized (BD Phosflow™ Perm Buffer III, Cat. No. 558050) on ice for at least 30 minutes, and then stained with Alexa Fluor® 647 Mouse anti-IGF-1 Receptor (CD221) (pY1131, Cat. No. 558588).  Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
      Product Details
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      BD Phosflow™
      IGF1R; IGF-I receptor; IGFIR; IGFR; Insulin-like growth factor 1 receptor
      Human (QC Testing), Mouse,Rat (Predicted)
      Mouse BALB/c IgG1, κ
      Phosphorylated Human IGF-1 Receptor
      Intracellular staining (flow cytometry) (Routinely Tested)
      20 µl
      AB_647151
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
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      Product Notices

      1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
      5. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
      6. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
      7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      8. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      558588 Rev. 2
      Antibody Details
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      K74-218

      Insulin-like growth factor-1 (IGF-1) receptor, or CD221, is a receptor tyrosine kinase that has high affinity for IGF-1 and low affinity for insulin and IGF-2.  Each transmembrane protein complex is formed from a pair of 1367 amino-acid precursor proteins.  After the 30 amino-acid signal sequences are removed, the paired polypeptides are cleaved to derive an α and β chain from each.  The two extracellular α chains form the ligand-binding domain, while the transmembrane-and-intracellular β chains carry the kinase activity.  Upon stimulation by its ligand, IGF-1 receptor autophosphorylates multiple tyrosine sites in its β chains and phosphorylates other signaling mediators that regulate cell growth, development, and neoplastic transformation.  

      The K74-218 monoclonal antibody recognizes the phosphorylated tyrosine 1131 (pY1131) in the activation loop of IGF-1 receptor's kinase domain.  The orthologous phosphorylation sites in mouse and rat IGF-1 receptor are Y1133 and Y1132, respectively.

      558588 Rev. 2
      Format Details
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      Alexa Fluor™ 647
      Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor™ 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 670-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      Alexa Fluor™ 647
      Red 627-640 nm
      653 nm
      669 nm
      558588 Rev.2
      Citations & References
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      View product citations for antibody "558588" on CiteAb

      Development References (4)

      1. Brodt P, Fallavollita L, Khatib A-M, Samani AA, Zhang, D. Cooperative regulation of the invasive and metastatic phenotypes by different domains of the type I insulin-like growth factor receptor β subunit. J Biol Chem. 2001; 276(36):33608-33615. (Biology). View Reference
      2. Dufresne AM, Smith RJ. The adapter protein Grb10 is an endogenous negative regulator of insulin-like growth factor signaling. Endocrinology. 2005. (Biology).
      3. Mitsiades CS, Mitsiades NS, McMullan CJ, et al. Inhibition of the insulin-like growth factor receptor-1 tyrosine kinase activity as a therapeutic strategy for multiple myeloma, other hematologic malignancies, and solid tumors. Cancer Cell. 2004; 5:221-230. (Biology). View Reference
      4. Ullrich A, Gray A, Tam AW, et al. Insulin-like growth factor I receptor primary structure: comparison with insulin receptor suggests structural determinants that define functional specificity. EMBO J. 1986; 5(10):2503-2512. (Biology).
      View All (4) View Less
      558588 Rev. 2

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.