Skip to main content Skip to navigation
Alexa Fluor™ 488 Rat Anti-Mouse CD206
Alexa Fluor™ 488 Rat Anti-Mouse CD206
Analysis of CD206 Expression by Thioglycolate-elicited Peritoneal Exudate Cells (Thio PECs)    Surface CD206 Expression - Panel 1: Thio PECs were stained with Alexa Fluor™ 647 Rat Anti-Mouse CD107b antibody (Cat. No. 564843) and with either Alexa Fluor™ 488 Rat IgG2a, κ Isotype Control (Cat. No. 557676;Top Plot) or Alexa Fluor™ 488 Rat Anti-Mouse CD206 antibody (Cat. No. 568806/568807; Bottom Plot) at 0.5 μg/test. DAPI Solution (Cat. No. 564907) was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD206 (or Ig Isotype control staining) versus CD107b was derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) cells.    Surface and Intracellular CD206 Expression - Panel 2: Thio PECs were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655), permeabilized with BD Perm/Wash™ Buffer (Cat. No. 554723) and stained as previously described at 0.5 µg/test. The bivariate plots showing the correlated expression of CD206 (or Ig Isotype control staining) versus CD107b were derived from gated events with the forward and side light-scatter characteristics of intact cells.    Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific and were generated in separate experiments.
Analysis of CD206 Expression by Thioglycolate-elicited Peritoneal Exudate Cells (Thio PECs)    Surface CD206 Expression - Panel 1: Thio PECs were stained with Alexa Fluor™ 647 Rat Anti-Mouse CD107b antibody (Cat. No. 564843) and with either Alexa Fluor™ 488 Rat IgG2a, κ Isotype Control (Cat. No. 557676;Top Plot) or Alexa Fluor™ 488 Rat Anti-Mouse CD206 antibody (Cat. No. 568806/568807; Bottom Plot) at 0.5 μg/test. DAPI Solution (Cat. No. 564907) was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD206 (or Ig Isotype control staining) versus CD107b was derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) cells.    Surface and Intracellular CD206 Expression - Panel 2: Thio PECs were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655), permeabilized with BD Perm/Wash™ Buffer (Cat. No. 554723) and stained as previously described at 0.5 µg/test. The bivariate plots showing the correlated expression of CD206 (or Ig Isotype control staining) versus CD107b were derived from gated events with the forward and side light-scatter characteristics of intact cells.    Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific and were generated in separate experiments.
Product Details
Down Arrow Up Arrow


BD Pharmingen™
CD206; MMR; Mrc1; macrophage mannose receptor 1
Mouse (QC Testing)
Rat WI, also known as Wistar (outbred) IgG2a, κ
Mouse CD206 Recombinant Protein
Flow cytometry (Routinely Tested)
0.2 mg/ml
17533
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  3. An isotype control should be used at the same concentration as the antibody of interest.
  4. Alexa Fluor® 488 fluorochrome emission is collected at the same instrument settings as for fluorescein isothiocyanate (FITC).
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. This product is provided under an intellectual property license between Life Technologies Corporation and BD Businesses. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Cell Analysis Business Unit Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.
  8. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  9. Alexa Fluor™ is a trademark of Life Technologies Corporation.
568807 Rev. 1
Antibody Details
Down Arrow Up Arrow
Y17-505

The Y17-505 monoclonal antibody specifically binds to CD206 which is also known as the Macrophage mannose receptor (MMR, MR) or Mannose receptor, C type 1 (Mrc1). CD206 is a type I transmembrane glycoprotein of approximately 175 kDa that belongs to the C-type lectin superfamily. It is expressed at the cell surface and intracellularly by macrophages, Langerhans cells, dendritic cells, and endothelial cells within hepatic and lymphoid tissues. This pattern recognition receptor binds to endogenous and microbial glycoconjugates containing mannose, fucose, or N-acetylglucosamine through its C-type lectin-like carbohydrate recognition domains (CRD). CD206 also contains a cysteine-rich domain that enables binding to sulfated carbohydrate antigens. This receptor enables macrophages and other specialized cells to maintain tissue homeostasis as well as to internalize microbes or their components by phagocytosis or endocytosis. CD206 thus plays important roles in mediating innate immunity, eg, enabling phagocytosis, as well as in processing and presenting antigens for the generation and expression of adaptive immunity. Moreover, CD206 has been associated with leucocyte homing and cancer cell metastasis.

568807 Rev. 1
Format Details
Down Arrow Up Arrow
Alexa Fluor™ 488
Alexa Fluor™ 488 Dye is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 517-nm. Alexa Fluor™ 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520-nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 488
Blue 488 nm
494 nm
517 nm
568807 Rev.1
Citations & References
Down Arrow Up Arrow

Development References (6)

  1. Akbarshahi H, Menzel M, Posaric Bauden M, Rosendahl A, Andersson R. Enrichment of murine CD68+ CCR2+ and CD68+ CD206+ lung macrophages in acute pancreatitis-associated acute lung injury. PLoS ONE. 2012; 7(10):e42654. (Biology). View Reference
  2. Burgdorf S, Lukacs-Kornek V, Kurts C. The mannose receptor mediates uptake of soluble but not of cell-associated antigen for cross-presentation. J Immunol. 2006; 176(11):6770-6776. (Biology). View Reference
  3. Marttila-Ichihara F, Turja R, Miiluniemi M, et al. Macrophage mannose receptor on lymphatics controls cell trafficking. Blood. 2008; 112(1):64-72. (Biology). View Reference
  4. McKenzie EJ, Taylor PR, Stillion RJ, et al. J Immunol. 2007; 178(8):4975-4983. (Biology). View Reference
  5. Rybalko V, Hsieh PL, Merscham-Banda M, Suggs LJ, Farrar RP. The Development of Macrophage-Mediated Cell Therapy to Improve Skeletal Muscle Function after Injury.. PLoS One. 2015; 10(12):e0145550. (Biology). View Reference
  6. Zamze S, Martinez-Pomares L, Jones H, et al. Recognition of bacterial capsular polysaccharides and lipopolysaccharides by the macrophage mannose receptor. J Biol Chem. 2002; 277(44):41613-41623. (Biology). View Reference
View All (6) View Less
568807 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.