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      FITC Hamster Anti-Mouse Bcl-2 Set

      BD Pharmingen™ FITC Hamster Anti-Mouse Bcl-2 Set

      (RUO)
      FITC Hamster Anti-Mouse Bcl-2 Set
      Flow cytometric analysis of Bcl-2 expression on permeabilized M1 mouse myelobast cells. M1 mouse myeloblast cells (ATCC TIB-191) were permeabilized with Fixation and Permeabilization Solution (Cat. No. 554722) and washed twice with Perm/Wash Buffer (Cat. No. 554723).  Permeabilized cells were stained with either FITC Hamster Anti-Mouse Bcl-2 (Cat. No. 554221, component51-15024X, filled histogram) or FITC Hamster IgG Isotype Control (Cat. No. 554221, component 51-66994X, open histogram). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable cells. Flow cytometry was performed on a BD FACScan™ system.  
      FITC Hamster Anti-Mouse Bcl-2 Set FITC Hamster Anti-Mouse Bcl-2 Set
      Flow cytometric analysis of Bcl-2 expression on permeabilized M1 mouse myelobast cells. M1 mouse myeloblast cells (ATCC TIB-191) were permeabilized with Fixation and Permeabilization Solution (Cat. No. 554722) and washed twice with Perm/Wash Buffer (Cat. No. 554723).  Permeabilized cells were stained with either FITC Hamster Anti-Mouse Bcl-2 (Cat. No. 554221, component51-15024X, filled histogram) or FITC Hamster IgG Isotype Control (Cat. No. 554221, component 51-66994X, open histogram). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable cells. Flow cytometry was performed on a BD FACScan™ system.  
      Product Details
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      BD Pharmingen™
      Mouse (QC Testing)
      Intracellular staining (flow cytometry) (Routinely Tested), Immunofluorescence, Immunohistochemistry-frozen, Immunoprecipitation, Western blot (Reported)
      RUO
      AB_395312


      Description

      Programmed cell death (apoptosis) is a normal physiologic process which occurs during embryonic development as well as in maintenence of tissue homeostasis. The apoptotic program is characterized by certain morphological features. These include changes in the plasma membrane such as loss of membrane asymmetry and attachment, a condensation of the cytoplasm and nucleus, and internucleosomal cleavage of DNA. In the final stages, the dying cells become fragmented into "apoptotic bodies" which are rapidly eliminated by phagocytic cells without eliciting significant inflammatory damage to surrounding cells. Members of the Bcl-2 family play a major role in regulating the cellular response to apoptotic signals. Bcl-2 is considered to be a novel proto-oncogene because it blocks apoptosis in many cell types. Bcl-2 is thought to provide selective survival advantage for cells by blocking apoptosis and thus may contribute to tumorigenesis. Bcl-2 is a 26 kD intracellular, integral membrane protein found primarily in the nuclear envelope, endoplasmic reticulum and outer mitochondrial membrane.

      Clone 3F11 reacts with mouse Bcl-2. It does not cross-react with human Bcl-2. Gel purified recombinant mouse Bcl-2 protein was used as immunogen.

      Clone A19-3 is an Armenian hamster IgG isotype control which is specific for the hapten trinitrophenol (TNP). This hapten is not expressed on human cells or human cell lines. The solutions are free of unconjugated antibody. The 3F11 and A19-3 FITC conjugates are matched in F/P ratios. The optimum F/P ratio was experimentally determined by flow cytometric analysis.

      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
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      Product Notices

      1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      2. This antibody has been optimized and preassayed with its matched isotype control to be used at the recommended volume of 20 ul/test. Titration of the reagents or substituting with other (non-matched) isotype control is NOT recommended.
      3. An isotype control should be used at the same concentration as the antibody of interest.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      6. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf.
      7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      8. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      554221 Rev. 6
      Components
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      Description Quantity/Size Part Number EntrezGene ID
      FITC Hamster Anti-Mouse Bcl-2 100 Tests (1 ea) 51-15024X N/A
      FITC Hamster IgG Isotype Control 100 Tests (1 ea) 51-66994X N/A
      554221 Rev. 6
      Citations & References
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      Development References (9)

      1. Alexander-Miller MA, Derby MA, Sarin A, Henkart PA, Berzofsky JA. Supraoptimal peptide-major histocompatibility complex causes a decrease in bc1-2 levels and allows tumor necrosis factor alpha receptor II-mediated apoptosis of cytotoxic T lymphocytes. J Exp Med. 1998; 188(8):1391-1399. (Clone-specific: Western blot). View Reference
      2. Krajewski S, Tanaka S, Takayama S, Schibler MJ, Fenton W, Reed JC. Investigation of the subcellular distribution of the bcl-2 oncoprotein: residence in the nuclear envelope, endoplasmic reticulum, and outer mitochondrial membranes. Cancer Res. 1993; 53(19):4701-4714. (Biology). View Reference
      3. Novack DV, Korsmeyer SJ. Bcl-2 protein expression during murine development. Am J Pathol. 1994; 145(1):61-73. (Clone-specific: Immunohistochemistry, Western blot). View Reference
      4. Oltvai ZN, Milliman CL, Korsmeyer SJ. Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death. Cell. 1993; 74(4):609-619. (Clone-specific: Immunoprecipitation). View Reference
      5. Reed JC, Tsujimoto Y, Alpers JD, Croce CM, Nowell PC. Regulation of bcl-2 proto-oncogene expression during normal human lymphocyte proliferation. Science. 1987; 236(4806):1295-1299. (Biology). View Reference
      6. Veis DJ, Sentman CL, Bach EA, Korsmeyer SJ. Expression of the Bcl-2 protein in murine and human thymocytes and in peripheral T lymphocytes. J Immunol. 1993; 151(5):2546-2554. (Immunogen: Flow cytometry, Fluorescence microscopy, Immunohistochemistry, Western blot). View Reference
      7. Veis DJ, Sorenson CM, Shutter JR, Korsmeyer SJ. Bcl-2-deficient mice demonstrate fulminant lymphoid apoptosis, polycystic kidneys, and hypopigmented hair. Cell. 1993; 75(2):229-240. (Clone-specific: Western blot). View Reference
      8. Williams GT. Programmed cell death: apoptosis and oncogenesis. Cell. 1991; 65(7):1097-1098. (Biology). View Reference
      9. Yang J, Liu X, Bhalla K, et al. Prevention of apoptosis by Bcl-2: release of cytochrome c from mitochondria blocked. Science. 1997; 275(5303):1129-1132. (Biology). View Reference
      View All (9) View Less
      554221 Rev. 6

      Please refer to Support Documents for Quality Certificates

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.