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BD Transduction Laboratories™ Purified Mouse Anti-SNX1
Clone 51/SNX1 (RUO)
Western blot analysis of SNX1 on HeLa lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of SNX1.
Immunofluorescence staining of HeLa cells.
Western blot analysis of SNX1 on HeLa lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of SNX1.
Immunofluorescence staining of HeLa cells.
Product Details
BD Transduction Laboratories™
Human (QC Testing)
Mouse IgG1
Human SNX1 aa. 1-108
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
74 kDa
250 µg/ml
AB_398942
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Companion Products
HRP Goat Anti-Mouse Ig RUO
Size
1 ML
Cat No.
554002
FITC Goat Anti-Mouse Ig RUO
Size
0.5 mg
Cat No.
554001
611482 Rev. 1
Antibody Details
51/SNX1
Biological processes such as transmembrane signaling and receptor mediated endocytosis revolve around the function of cell surface receptors. A network of molecular machinery directs the intracellular trafficking of receptors during their biosynthesis and mediates signaling downstream of receptors. The sorting nexins (SNX1, SNX1A, SNX2, SNX3, and SNX4) are a family of intracellular proteins that are thought to direct the sorting of receptor proteins. The SNX proteins contain a conserved 100 amino acid region termed the phox homology (PX) domain and are part of a family of hydrophilic proteins which includes S. cerevisiae proteins that function in protein sorting. SNX1, SNX2, and SNX4 associate predominantly with membranes and bind transmembrane receptors such as those for EGF, PDGF, and insulin. SNX1 directs the EGF receptor to the lysosomes for degradation. SNX2 forms homomeric complexes and heteromeric complexes with SNX1, SNX1A, and SNX4. These complexes are thought to be necessary for efficient protein sorting. Thus, SNX1 and other sorting nexins are thought to play important roles in the specificity of protein trafficking to and from the plasma membrane.
611482 Rev. 1
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611482 Rev.1
Citations & References
View product citations for antibody "611482" on CiteAb
Development References (4)
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Cozier GE, Carlton J, McGregor AH. The phox homology (PX) domain-dependent, 3-phosphoinositide-mediated association of sorting nexin-1 with an early sorting endosomal compartment is required for its ability to regulate epidermal growth factor receptor degradation. J Biol Chem. 2002; 277(50):48730-48736. (Clone-specific: Immunofluorescence, Western blot). View Reference
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Haft CR, de la Luz Sierra M, Barr VA, Haft DH, Taylor SI. Identification of a family of sorting nexin molecules and characterization of their association with receptors. Mol Cell Biol. 1998; 18(12):7278-7287. (Biology). View Reference
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Kurten RC, Cadena DL, Gill GN. Enhanced degradation of EGF receptors by a sorting nexin, SNX1. Science. 1996; 272(5264):1008-1010. (Biology). View Reference
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Strehlow D, Jelaska A, Strehlow K, Korn JH. A potential role for protease nexin 1 overexpression in the pathogenesis of scleroderma. J Clin Invest. 1999; 103(8):1179-1190. (Biology). View Reference
611482 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.