Description
The purpose of this kit is to provide the necessary reagents to prepare full length Mouse TCR and BCR libraries and is compatible with both Whole Transcriptome Analysis (WTA) and Targeted mRNA assays. In addition to this kit, an mRNA library preparation kit will be required, either the WTA amplification kit (cat no. 633801) or the Targeted mRNA & AbSeq Amp kit (cat no. 633774) with a targeted primer panel like the Mouse Immune Response panel (cat no. 633753). Cell capture (cat no. 664887 and 633733) and cDNA (cat no. 633773) reagents will also be needed. Learn more about the intended use of this kit and the associated protocol from the BD Rhapsody™ Single-Cell Analysis System user guide.
| Description | Quantity/Size | Part Number | EntrezGene ID |
|---|---|---|---|
| TSO | 1 Each (1 ea) | 91-1197 | N/A |
| 1M MgCl2 | 1 Each (1 ea) | 91-1198 | N/A |
| Hybridization Buffer | 1 Each (4 ea) | 91-1199 | N/A |
| Mouse TCR N1 Primer | 1 Each (1 ea) | 91-1212 | N/A |
| Mouse TCR N2 Primer | 1 Each (1 ea) | 91-1213 | N/A |
| Mouse BCR N1 Primer | 1 Each (1 ea) | 91-1214 | N/A |
| Mouse BCR N2 Primer | 1 Each (1 ea) | 91-1215 | N/A |
| TCR/BCR Universal Oligo N1 | 1 Each (1 ea) | 91-1204 | N/A |
| TCR/BCR Universal Oligo N2 | 1 Each (1 ea) | 91-1205 | N/A |
| TCR/BCR Extension Buffer | 1 Each (1 ea) | 91-1206 | N/A |
| TCR/BCR Extension Enzyme | 1 Each (1 ea) | 91-1207 | N/A |
| TCR/BCR Extension Primers | 1 Each (1 ea) | 91-1208 | N/A |
| Nuclease-Free Water | 1 Each (2 ea) | 650000076 | N/A |
| Bead RT/PCR Enhancer | 1 Each (1 ea) | 91-1082 | N/A |
| PCR MasterMix | 1 Each (1 ea) | 91-1083 | N/A |
| Elution Buffer | 1 Each (2 ea) | 91-1084 | N/A |
| dNTP | 1 Each (2 ea) | 650000077 | N/A |
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.