Preparation And Storage
Recommended Assay Procedures
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
The p90[rsk] (Rsk) and p70[s6k] kinases were first identified based on their ability to phosphorylate the 40S ribosomal protein S6 in vitro. Both of these enzymes are differentially regulated by serine/threonine phosphorylation in response to mitogenic stimulation. ERK1 and ERK2 have been shown to regulate Rsk activity. Once activated by this phosphorylation, a significant amount of Rsk can be found in the nucleus, suggesting that it has a role in nuclear signaling events. The regulation of nuclear Rsk and ERK activities by growth factors is coordinated with the induction of several early response genes. Rsk has also been shown to be activated by ionizing radiation, presumably through an activated MAP kinase. Studies in Xenopus oocytes and mouse NIH/3T3 cells indicate that inactive Rsk and ERK2 exist in a complex of approximately 110kDa. Upon phosphorylation of Rsk and ERK2, the heterodimer dissociates and at least a portion of these activated kinases translocate to the nucleus.
Development References (5)
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Chung J, Pelech SL, Blenis J. Mitogen-activated Swiss mouse 3T3 RSK kinases I and II are related to pp44mpk from sea star oocytes and participate in the regulation of pp90rsk activity. Proc Natl Acad Sci U S A. 1991; 88(11):4981-4985. (Biology). View Reference
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Hsiao KM, Chou SY, Shih SJ, Ferrell JE Jr. Evidence that inactive p42 mitogen-activated protein kinase and inactive Rsk exist as a heterodimer in vivo. Proc Natl Acad Sci U S A. 1994; 9(12):5480-5484. (Biology). View Reference
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Majka M, Janowska-Wieczorek A, Ratajczak J. Stromal-derived factor 1 and thrombopoietin regulate distinct aspects of human megakaryopoiesis. Blood. 2000; 96(13):4142-4151. (Clone-specific: Western blot). View Reference
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Moor AN, Gan XT, Karmazyn M, Fliegel L. Activation of Na+/H+ exchanger-directed protein kinases in the ischemic and ischemic-reperfused rat myocardium. J Biol Chem. 2001; 276(19):16113-16122. (Clone-specific: Western blot). View Reference
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Morrione A, Navarro M, Romano G. The role of the insulin receptor substrate-1 in the differentiation of rat hippocampal neuronal cells. Oncogene. 2001; 20(35):4842-4852. (Clone-specific: Western blot). View Reference
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