Preparation And Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Companion Products
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Rap1 is a member of the large Ras superfamily of low molecular weight GTP/GDP binding proteins. Like Ras, the Rap proteins cycle between a GDP-bound inactive form and a GTP-bound active form. Since Ras and Rap have the same amino acid sequence in their putative effector domain (aa. 32-40), it seems likely that they perform either similar or antagonistic functions. Rap1A and Rap1B are highly homologous proteins, differing in only 9 of their 184 amino acids. Overexpression of Rap1A (also known as Krev-1) causes reversion of the phenotype of a Ki-Ras-transformed cell line. In vitro, Rap1 can compete efficiently with p21ras for interaction with Ras-GAP. Though they appear to have similar activities, Rap1 and Ras differ in their cellular localization. Ras is found on the inner surface of the plasma membrane while Rap1 is associated with the Golgi.
This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Development References (5)
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Larson MK, Chen H, Kahn ML, et al. Identification of P2Y12-dependent and -independent mechanisms of glycoprotein VI-mediated Rap1 activation in platelets. Blood. 2003; 101(4):1409-1415. (Biology: Western blot). View Reference
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Okada S, Pessin JE. Insulin and epidermal growth factor stimulate a conformational change in Rap1 and dissociation of the CrkII-C3G complex. J Biol Chem. 1997; 272(45):28179-28182. (Biology: Immunoprecipitation, Western blot). View Reference
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Wu C, Lai CF, Mobley WC. Nerve growth factor activates persistent Rap1 signaling in endosomes. J Neurosci. 2001; 21(15):5406-5416. (Biology: Immunofluorescence, Western blot). View Reference
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Xing L, Ge C, Zeltser R, Maskevitch G, Mayer BJ, Alexandropoulos K. c-Src signaling induced by the adapters Sin and Cas is mediated by Rap1 GTPase. Mol Cell Biol. 2000; 20(19):7363-7377. (Biology: Western blot). View Reference
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Yamamoto T, Kaibuchi K, Mizuno T, Hiroyoshi M, Shirataki H, Takai Y. Purification and characterization from bovine brain cytosol of proteins that regulate the GDP/GTP exchange reaction of smg p21s, ras p21-like GTP-binding proteins. J Biol Chem. 1990; 265(27):16626-16634. (Biology). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.