Preparation And Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
The JAK family of receptor-associated protein kinases is directly involved in interferon (IFN) response pathways. The JAK family contains at least three members: JAK1, JAK2, and Tyk2. Each protein is approximately 130 kDa and contains a C-terminal tyrosine kinase domain, an adjacent kinase or kinase-related domain, and five other domains that are highly conserved among family members. In several human and murine cell lines, JAK1 is rapidly tyrosine phosphorylated in response to IFN-α and IFN-γ. JAK1 is required for the phosphorylation of the transcription factor Stat1 (p91), in response to IFNs-α or IFN-γ. JAK1 is also necessary for the efficient phophorylation of Stat2 (p113) in response to IFN-α and for the phosphorylation of Tyk2 or JAK2 in response to IFNs-α or IFN-γ, respectively.
Development References (5)
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Blesofsky WA, Mowen K, Arduini RM. Regulation of STAT protein synthesis by c-Cbl. Oncogene. 2001; 20(50):7326-7333. (Clone-specific: Immunoprecipitation, Western blot). View Reference
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Kawazoe Y, Naka T, Fujimoto M. Signal transducer and activator of transcription (STAT)-induced STAT inhibitor 1 (SSI-1)/suppressor of cytokine signaling 1 (SOCS1) inhibits insulin signal transduction pathway through modulating insulin receptor substrate 1 (IRS-1) phosphorylation. J Exp Med. 2001; 193(2):263-269. (Clone-specific: Western blot). View Reference
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Kopantzev Y, Heller M, Swaminathan N, Rudikoff S. IL-6 mediated activation of STAT3 bypasses Janus kinases in terminally differentiated B lineage cells. Oncogene. 2002; 21(44):6791-6800. (Clone-specific: Immunoprecipitation, Western blot). View Reference
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Muller M, Briscoe J, Laxton C. The protein tyrosine kinase JAK1 complements defects in interferon-alpha/beta and -gamma signal transduction. Nature. 1993; 366(6451):129-135. (Biology). View Reference
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Nicholson SE, Oates AC, Harpur AG, Ziemiecki A, Wilks AF, Layton JE. Tyrosine kinase JAK1 is associated with the granulocyte-colony-stimulating factor receptor and both become tyrosine-phosphorylated after receptor activation. Proc Natl Acad Sci U S A. 1994; 91(8):2985-2988. (Biology). View Reference
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.