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Purified Mouse Anti-Cox-2
Purified Mouse Anti-Cox-2
Western blot analysis of Cox-2 on a lysate from mouse macrophages treated with IFNγ and LPS.  Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the Mouse Anti-Cox-2 antibody.
Purified Mouse Anti-Cox-2
Immunohistochemical staining of neurons and endothelial cells from blood vessels (formalin-fixed, citrate buffer pre-treatment, 10X).
Purified Mouse Anti-Cox-2
Immunofluoresence staining of mouse macrophages.
Western blot analysis of Cox-2 on a lysate from mouse macrophages treated with IFNγ and LPS.  Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the Mouse Anti-Cox-2 antibody.
Immunohistochemical staining of neurons and endothelial cells from blood vessels (formalin-fixed, citrate buffer pre-treatment, 10X).
Immunofluoresence staining of mouse macrophages.
Product Details
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BD Transduction Laboratories™
PGHS-2, Cyclooxygenase-2
Mouse (QC Testing), Human,Chicken (Tested in Development)
Mouse IgG1
Rat Cox-2 aa. 368-604
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry (Tested During Development), Immunoprecipitation (Not Recommended)
70 kDa
250 µg/ml
AB_397603
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
610203 Rev. 1
Antibody Details
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33/Cox-2

Cyclooxygenase (Cox) is also known as prostaglandin H synthase or PGH synthase (E.C. 1.14.99.1). It catalyzes the conversion of arachidonate to prostaglandin H2 (PGH2), the precursor of PGE2, PGF2α, PGD2, prostacyclin, and thromboxane A2. Cox actually has two different enzymatic activities: a cyclooxygenase that mediates the formation of PGG2 from oxygen and  arachidonate and a hydroperoxidase that catalyzes a reduction of PGG2 yielding PGH2. Two Cox genes, Cox-1 and Cox-2, have been isolated in several species. A 4kb mRNA encodes the 604 amino acid Cox-2 protein. The two human Cox isoenzymes are 61% identical in amino acid composition with the active sites being highly conserved. Cox-2 mRNA and protein levels are induced by serum, lipopolysaccharides, growth factors, human chorionic gonadotropin and phorbol  testers in various mammalian cell types. It has been shown that interleukin-1α (IL-1α) induces increased levels of Cox-2 mRNA and protein in human endothelial cells. The sustained increase in Cox-2 is apparently due (at least in part) to IL-1α increasing the stability of Cox-2 mRNA. This type of regulatory mechanism may play an important role in chronic inflammatory conditions.

610203 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610203 Rev.1
Citations & References
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View product citations for antibody "610203" on CiteAb

Development References (5)

  1. Giroux M, Descoteaux A. Cyclooxygenase-2 expression in macrophages: modulation by protein kinase C-alpha. J Immunol. 2000; 167(7):3985-3991. (Biology: Western blot). View Reference
  2. Hla T, Neilson K. Human cyclooxygenase-2 cDNA. Proc Natl Acad Sci U S A. 1992; 89(16):7384-7388. (Biology). View Reference
  3. Marrogi A, Pass HI, Khan M, Metheny-Barlow LJ, Harris CC, Gerwin BI. Human mesothelioma samples overexpress both cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (NOS2): in vitro antiproliferative effects of a COX-2 inhibitor. Cancer Res. 2000; 20(18):6862-6867. (Biology: Immunofluorescence, Immunohistochemistry). View Reference
  4. Shiotani H, Denda A, Yamamoto K. Increased expression of cyclooxygenase-2 protein in 4-nitroquinoline-1-oxide-induced rat tongue carcinomas and chemopreventive efficacy of a specific inhibitor, nimesulide. Cancer Res. 2001; 61(4):451-456. (Biology: Immunohistochemistry, Western blot). View Reference
  5. Xie QW, Cho HJ, Calaycay J, et al. Cloning and characterization of inducible nitric oxide synthase from mouse macrophages. Science. 1992; 256(5054):225-228. (Biology). View Reference
View All (5) View Less
610203 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.