Description
D-Luciferin (sodium salt), also known as beetle luciferin or (D-(-)-2-(6'-hydroxy-2'-benzothiazolyl)-thiazoline-4-carboxylic acid), has a formula weight of 302.30 and is a synthetically made substrate which can be catalyzed by firefly luciferase (enzymatic reaction shown below). D-Luciferin is lyophilized in the form of a monosodium salt and has been purified by HPLC to remove dehydroluciferin and L-Luciferin, both of which are competitive inhibitors of firefly luciferase. It is important to note, however, that D-Luciferin can form dehydroluciferin in the presence of ultraviolet light and/or moisture. For this reason, prior to reconstitution, store in the dark at -20°C.
Preparation And Storage
Store lyophilized D-Luciferin at -20 °C in the dark.Reconstitute lyophilized D-Luciferin with deionized water and bring the solution to a pH of 6.0 - 6.3 with HCl/NaOH. At a pH greater than 6.3, D-Luciferin may undergo a slow racemization from the D- to the L-isomer.After reconstitution, store at 4 °C and protected from light for up to 2 weeks or alternatively, store at -20 °C for up to 1 year [aliquoted and protected from light].
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.