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      RY586 Mouse Anti-Human CD156c
      Product Details
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      BD OptiBuild™
      ADAM 10; AD10; ADA10; MADM; KUZ; Kuzbanian protein homolog ; HsT18717; RAK
      Human (Tested in Development)
      Mouse BALB/c IgG1, κ
      Human Jurkat Cell Line
      Flow cytometry (Qualified)
      0.2 mg/ml
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.
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      Recommended Assay Procedures

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

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      Product Notices

      1. Researchers should determine the optimal concentration of this reagent for their individual applications.
      2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
      3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      4. An isotype control should be used at the same concentration as the antibody of interest.
      5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      6. CF™ is a trademark of Biotium, Inc.
      7. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      9. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      10. Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
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      753558 Rev. 1
      Antibody Details
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      11G2

      The 11G2 monoclonal antibody specifically binds to CD156c which is also known as Disintegrin and metalloproteinase domain-containing protein 10 (ADAM10). CD156c is a ~70 kDa type I transmembrane glycoprotein that belongs to the ADAM family. It is widely expressed on hematopoietic and non-hematopoietic cells. CD156c is expressed on the plasma membrane, as well as by membranes within intracellular compartments and exosomes. CD156c serves as a broadly-reactive endopeptidase that cleaves membrane-bound proteins in a process known as ectodomain shedding or release. It can cleave transmembrane molecules into soluble forms and thereby regulate the functions of a variety of different receptors and ligands that are involved in cellular signaling and adhesion. These include membrane TNF, CX3CL1, CXCL16, Ephrin-A2, Notch and Delta molecules, cadherins, CD23, CD44, IL-6 Receptor/CD126, CD171, and amyloid precursor protein. CD156c expression may be constitutive or induced, such as in inflamed tissues, including arthritic joints and in the nervous system. Dysregulated CD156c expression may be associated with certain cancers and Alzheimer's disease.

      753558 Rev. 1
      Format Details
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      RY586
      The BD Horizon RealYellow™ 586 (RY586) Dye is part of the BD family of yellow-green dyes. It is a small organic fluorochrome with an excitation maximum (Ex Max) at 565-nm and an emission maximum (Em Max) at 586-nm. Driven by BD innovation, RY586 can be used on both spectral and conventional cytometers and is designed to be excited by the Yellow-Green laser (561-nm) with minimal excitation by the 488-nm Blue laser. For conventional instruments equipped with a Yellow-Green laser (561-nm), RY586 can be used as an alternative to PE and we recommend using an optical filter centered near 586-nm (eg, a 586/15-nm bandpass filter). For spectral instruments equipped with a Yellow-Green laser (561-nm), it can be used in conjunction with PE. Compared to PE, RY586 is similar in brightness, minimal spillover into Blue detectors, and increased spillover into the 610/20-nm (PE-CF594) detector. Please ensure that your instrument configuration (lasers and optical filters) is appropriate for this dye.
      altImg
      RY586
      Yellow-Green 561 nm
      564 nm
      586 nm
      753558 Rev.1
      Citations & References
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      View product citations for antibody "753558" on CiteAb

      Development References (7)

      1. Arduise C, Abache T, Li L, et al. Tetraspanins regulate ADAM10-mediated cleavage of TNF-alpha and epidermal growth factor.. J Immunol. 2008; 181(10):7002-13. (Immunogen: Flow cytometry, Fluorescence microscopy, Immunoaffinity chromatography, Immunofluorescence, Immunoprecipitation, Western blot). View Reference
      2. Huovila AP, Turner AJ, Pelto-Huikko M, Kärkkäinen I, Ortiz RM. Shedding light on ADAM metalloproteinases.. Trends Biochem Sci. 2005; 30(7):413-22. (Biology). View Reference
      3. Pruessmeyer J, Ludwig A. The good, the bad and the ugly substrates for ADAM10 and ADAM17 in brain pathology, inflammation and cancer.. Semin Cell Dev Biol. 2009; 20(2):164-74. (Biology). View Reference
      4. Saftig P, Lichtenthaler SF. The alpha secretase ADAM10: A metalloprotease with multiple functions in the brain.. Prog Neurobiol. 2015; 135:1-20. (Biology). View Reference
      5. Stoeck A, Keller S, Riedle S, et al. A role for exosomes in the constitutive and stimulus-induced ectodomain cleavage of L1 and CD44.. Biochem J. 2006; 393(Pt 3):609-18. (Clone-specific: Flow cytometry, Immunoprecipitation, Western blot). View Reference
      6. Zola H, Swart B, Banham A, et al. CD molecules 2006--human cell differentiation molecules.. J Immunol Methods. 2007; 319(1-2):1-5. (Clone-specific: Flow cytometry). View Reference
      7. Zola H, Swart B, Nicholson I, Voss E. CD156c. In: Zola H. Leukocyte and stromal cell molecules : the CD markers. Hoboken, N.J.: Wiley-Liss; 2007:288.
      View All (7) View Less
      753558 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.