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Purified Mouse Anti-Human CD209
Purified Mouse Anti-Human CD209

Immunohistochemistry of dendritic cells stained for CD209 (DC-SIGN). An acetone-fixed frozen section from human tonsil was stained with the Purified Mouse Anti-Human CD209 antibody. Dendritic cells expressing CD209 (DC-SIGN) can be identified by the intense brown labeling of their cell membranes (magnification 40X).

Immunohistochemistry of dendritic cells stained for CD209 (DC-SIGN). An acetone-fixed frozen section from human tonsil was stained with the Purified Mouse Anti-Human CD209 antibody. Dendritic cells expressing CD209 (DC-SIGN) can be identified by the intense brown labeling of their cell membranes (magnification 40X).

Product Details
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BD Pharmingen™
DC-SIGN
Human (QC Testing)
Mouse IgG2b, κ
Human Monocyte Derived DC Cells
Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), Immunohistochemistry-paraffin (Not Recommended)
31.25 µg/ml
AB_394120
Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Immunohistochemistry: This antibody is recommended to test for immunohistochemical staining on acetone-fixed frozen sections from either human spleen or tonsil and reported to detect dendritic cells. IHC of formalin-fixed paraffin embedded sections is not recommended. For optimal indirect immunohistochemical staining, the DCN46 antibody should be titrated (1:10 to 1:50 dilution) and visualized via a three-step staining procedure in combination with biotinylated polyclonal anti-mouse Ig (Cat. No. 550337) as the secondary antibody and Streptavidin-HRP (Cat. No. 550946) together with the DAB detection system (Cat. No. 550880). More conveniently, the anti-mouse Ig HRP detection kit (Cat. No. 551011) can be used which contains the biotinylated secondary antibody, antibody diluent, streptavidin-HRP and a DAB substrate for use in the staining procedure.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. This antibody has been developed for the immunohistochemistry application. However, a routine immunohistochemistry test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  7. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
551249 Rev. 8
Antibody Details
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DCN46

The DCN46 antibody specifically binds to dendritic cell-specific ICAM-3 grabbing nonintegrin (DC-SIGN or CD209), a type-II membrane protein of approximately 44 kDa with a mannose-binding C-type lectin domain.  It is highly expressed on dendritic cells in mucosal tissues.  Its sequence is identical to the HIV-1 envelope gp120-binding C-type lectin, and reports suggest that DC-SIGN binds to HIV-1 gp120 and effectively transmits infectious HIV-1 to resting T lymphocytes expressing CD4 and chemokine receptors.  The C-type lectin domain of DC-SIGN is also capable of binding other pathogenic viruses, bacteria, and parasites.  Reports also suggest that DC-SIGN enables the highly efficient migration of dendritic cells from blood into the tissues.  It can interact with ICAM-2, which has a similar sequence as ICAM-3, and is abundantly expressed on vascular and lymphoid endothelium.  Thus, DC-SIGN mediates dendritic cells rolling and transendothelial migration, and its interaction with ICAM-2 is essential to specific migratory functions of dendritic cells.

551249 Rev. 8
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
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Citations & References
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Development References (4)

  1. Geijtenbeek TBH, Kwon DS, Torensma R, et al. DC-SIGN, a dendritic cell-specific HIV-1 -binding protein that enhances trans-infection of T cells. Cell. 2000; 100(5):587-597. (Biology). View Reference
  2. Geijtenbeek TBH, Torensma R, van Vliet SJ, et al. Identification of DC-SIGN, a novel dendritic cell-specific ICAM-3 receptor that supports primary immune responses. Cell. 2000; 100(5):575-585. (Biology). View Reference
  3. Sallusto F, Cella M, Danieli C, Lanzavecchia A. Dendritic cells use macropinocytosis and the mannose receptor to concentrate macromolecules in the major histocompatibility complex class II compartment: downregulation by cytokines and bacterial products. J Exp Med. 1995; 182(2):389-400. (Immunogen). View Reference
  4. Steinman RM. DC-SIGN: a guide to some mysteries to dendritic cells. Cell. 2000; 100(5):491-494. (Biology). View Reference
View All (4) View Less
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Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.