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Purified Hamster Anti-Mouse CD11c
Purified Hamster Anti-Mouse CD11c
Flow cytometric analysis of CD11c expression on mouse splenocytes. C57BL/6 mouse splenocytes were stained with PE Mouse Anti-Mouse NK-1.1 (Cat. No. 557391/553165) and either Purified Hamster IgG Isotype Control (Cat. No. 553951; left panel) or Purified Hamster anti-Mouse CD11c antibody (Cat. No.553799/550283; right panel). Secondary staining was carried out with FITC Mouse Anti-Armenian and Syrian Hamster IgG Cocktail (Cat. No. 554011). The contour plots were derived from events with the forward and side light-scatter characteristics of viable splenocytes. Flow cytometric analysis was performed using a BD FACSCan™ flow cytometry system.
Flow cytometric analysis of CD11c expression on mouse splenocytes. C57BL/6 mouse splenocytes were stained with PE Mouse Anti-Mouse NK-1.1 (Cat. No. 557391/553165) and either Purified Hamster IgG Isotype Control (Cat. No. 553951; left panel) or Purified Hamster anti-Mouse CD11c antibody (Cat. No.553799/550283; right panel). Secondary staining was carried out with FITC Mouse Anti-Armenian and Syrian Hamster IgG Cocktail (Cat. No. 554011). The contour plots were derived from events with the forward and side light-scatter characteristics of viable splenocytes. Flow cytometric analysis was performed using a BD FACSCan™ flow cytometry system.
Product Details
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BD Pharmingen™
Cd11c; Itgax; Integrin alpha-X; Integrin αX; Cr4; Complement receptor 4
Mouse (QC Testing)
Armenian Hamster IgG1, λ2
C57BL/6 Mouse Intestinal Intraepithelial Lymphocytes
Flow cytometry (Routinely Tested), Immunohistochemistry-frozen, Immunoprecipitation (Reported), Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-zinc-fixed (Not Recommended)
0.5 mg/ml
16411
AB_395058
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

For IHC, we recommend the use of purified HL3 mAb in our special formulation for immunohistochemistry (Cat. No. 550283).

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf.
  5. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
553799 Rev. 2
Antibody Details
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HL3

The HL3 monoclonal antibody specifically binds to the integrin αx chain of gp150, 95 (CD11c/CD18). CD11c is expressed on dendritic cells, CD4- CD8+ intestinal intraepithelial lymphocytes (IEL) and some NK cells. It is upregulated on IEL and lymph-node T cells following in vivo activation. Cells of the monocyte/macrophage lineage have been reported to express low levels of CD11c. CD11c plays a role in binding of iC3b.

553799 Rev. 2
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
553799 Rev.2
Citations & References
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View product citations for antibody "553799" on CiteAb

Development References (8)

  1. Barclay NA, Brown MH, Birkeland ML, et al, ed. The Leukocyte Antigen FactsBook. San Diego, CA: Academic Press; 1997.
  2. Burt BM, Plitas G, Stableford JA, et al. CD11c identifies a subset of murine liver natural killer cells that responds to adenoviral hepatitis. J Leukoc Biol. 2008; 84(4):1039-1046. (Clone-specific). View Reference
  3. Fagarasan S, Muramatsu M, Suzuki K, Nagaoka H, Hiai H, Honjo T. Critical roles of activation-induced cytidine deaminase in the homeostasis of gut flora. Science. 2002; 298(5597):1424-1427. (Clone-specific: Fluorescence microscopy). View Reference
  4. Gao JX, Liu X, Wen J, et al. Differentiation of monocytic cell clones into CD8 alpha+ dendritic cells (DC) suggests that monocytes can be direct precursors for both CD8 alpha+ and CD8 alpha- DC in the mouse. J Immunol. 2003; 170(12):5927-5935. (Biology). View Reference
  5. Huleatt JW, Lefrançois L. Antigen-driven induction of CD11c on intestinal intraepithelial lymphocytes and CD8+ T cells in vivo.. J Immunol. 1995; 154(11):5684-93. (Immunogen: Immunoprecipitation). View Reference
  6. Larson RS, Springer TA. Structure and function of leukocyte integrins. Immunol Rev. 1990; 114:181-217. (Biology). View Reference
  7. Maraskovsky E, Brasel K, Teepe M, et al. Dramatic increase in the numbers of functionally mature dendritic cells in Flt3 ligand-treated mice: multiple dendritic cell subpopulations identified. J Exp Med. 1996; 184(5):1953-1962. (Biology). View Reference
  8. Pulendran B, Lingappa J, Kennedy MK, et al. Developmental pathways of dendritic cells in vivo: distinct function, phenotype, and localization of dendritic cell subsets in FLT3 ligand-treated mice. J Immunol. 1997; 159(5):2222-2231. (Clone-specific: Immunohistochemistry). View Reference
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553799 Rev. 2

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.