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      PE Rat Anti-Mouse CD3 Molecular Complex
      PE Rat Anti-Mouse CD3 Molecular Complex
      CD3 expression in spleen and thymus. BALB/c splenocytes were simultaneously stained with PE-conjugated mAb 17A2 and FITC-conjugated anti-mouse CD19 mAb 1D3 (Cat. No. 557398/553786, left panel). C3H/HeN thymocytes were stained with PE-conjugated 17A2 mAb (right panel, filled histogram) or unstained (right panel, empty histogram). Flow cytometry was performed on a BD FACScan™ flow cytometry system.
      PE Rat Anti-Mouse CD3 Molecular Complex
      CD3 expression in spleen and thymus. BALB/c splenocytes were simultaneously stained with PE-conjugated mAb 17A2 and FITC-conjugated anti-mouse CD19 mAb 1D3 (Cat. No. 557398/553786, left panel). C3H/HeN thymocytes were stained with PE-conjugated 17A2 mAb (right panel, filled histogram) or unstained (right panel, empty histogram). Flow cytometry was performed on a BD FACScan™ flow cytometry system.
      Product Details
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      BD Pharmingen™
      Mouse (QC Testing)
      Rat SD, also known as Sprague-Dawley (outbred) IgG2b, κ
      γδ TCR-positive T-T hybridoma D1
      Flow cytometry (Routinely Tested)
      0.2 mg/ml
      12501
      AB_395699
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      Product Notices

      1. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      555275 Rev. 8
      Antibody Details
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      17A2

      The 17A2 monoclonal antibody specifically binds to the T-cell receptor-associated CD3 complex that is expressed on many thymocytes and mature T lymphocytes. Plate-bound 17A2 antibody has been reported to induce IL-2 production by cultured T cells in the absence of accessory cells. The binding of the 17A2 antibody to T cells can be blocked by the anti-CD3e mAb 145-2C11 (Cat. No. 557306/553058/550275). This suggests that the 17A2 antibody recognizes an epitope of the CD3 epsilon chain. In vivo treatment with 17A2 antibody has been reported to partially deplete T lymphocytes and temporarily down-modulates CD3 expression on T cells.

      555275 Rev. 8
      Format Details
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      PE
      R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PE
      Yellow-Green 488 nm, 532 nm, 561 nm
      496 nm, 566 nm
      576 nm
      555275 Rev.8
      Citations & References
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      View product citations for antibody "555275" on CiteAb

      Development References (2)

      1. Miescher GC, Schreyer M, MacDonald HR. Production and characterization of a rat monoclonal antibody against the murine CD3 molecular complex. Immunol Lett. 1989; 23(2):113-118. (Biology: Induction). View Reference
      2. Mysliwietz J, Thierfelder S. Antilymphocytic antibodies and marrow transplantation. XII. Suppression of graft-versus-host disease by T-cell-modulating and depleting antimouse CD3 antibody is most effective when preinjected in the marrow recipient. Blood. 1992; 80(10):2661-2667. (Biology: Depletion). View Reference
      555275 Rev. 8

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.