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BD Pharmingen™ PE Mouse Anti-Human TCR Vα7.2
Clone OF-5A12 (RUO)
Two-color flow cytometric analysis of TCR Vα7.2 expression on human peripheral blood cells. Human whole blood was stained with BD Horizon™ BV421 Mouse Anti-Human CD3 antibody (Cat. No. 562426/562427) and either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; Left Plot) or PE Mouse Anti-Human TCR Vα7.2 antibody (Cat. No. 566739; Right Plot). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Pseudocolor density plots showing the correlated expression of CD3 versus TCR Vα7.2 (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software.
Two-color flow cytometric analysis of TCR Vα7.2 expression on human peripheral blood cells. Human whole blood was stained with BD Horizon™ BV421 Mouse Anti-Human CD3 antibody (Cat. No. 562426/562427) and either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; Left Plot) or PE Mouse Anti-Human TCR Vα7.2 antibody (Cat. No. 566739; Right Plot). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Pseudocolor density plots showing the correlated expression of CD3 versus TCR Vα7.2 (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software.
Two-color flow cytometric analysis of TCR Vα7.2 expression on human peripheral blood cells. Human whole blood was stained with BD Horizon™ BV421 Mouse Anti-Human CD3 antibody (Cat. No. 562426/562427) and either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; Left Plot) or PE Mouse Anti-Human TCR Vα7.2 antibody (Cat. No. 566739; Right Plot). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Pseudocolor density plots showing the correlated expression of CD3 versus TCR Vα7.2 (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software.
Two-color flow cytometric analysis of TCR Vα7.2 expression on human peripheral blood cells. Human whole blood was stained with BD Horizon™ BV421 Mouse Anti-Human CD3 antibody (Cat. No. 562426/562427) and either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; Left Plot) or PE Mouse Anti-Human TCR Vα7.2 antibody (Cat. No. 566739; Right Plot). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Pseudocolor density plots showing the correlated expression of CD3 versus TCR Vα7.2 (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software.
Product Details
BD Pharmingen™
TRAV7; TCR V alpha 7.2; T cell receptor alpha variable 7.2; TCRAV7.2
Human (QC Testing)
Mouse IgG1, κ
Human Va7.2-expressing T cell clones
Flow cytometry (Routinely Tested)
5 µl/test
AB_2869841
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 ML
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 ML
Cat No.
554657
Lysing Buffer RUO
Size
100 ML
Cat No.
555899
Lysing Solution 10X Concentrate IVD
Size
100 ML
Cat No.
349202
PE Mouse IgG1, κ Isotype Control RUO
Size
0.1 mg
Cat No.
554680
BV421 Mouse Anti-Human CD3 RUO
Size
25 Tests
Cat No.
562427
566739 Rev. 1
Antibody Details
OF-5A12
The OF-5A12 monoclonal antibody specifically recognizes the human variable alpha 7.2 region of the T cell receptor for antigen (TCR Vα7.2) that is joined with the Jα33 segment to form an invariant TCR which is characteristically expressed by mucosal-associated invariant T cells (MAIT cells). MAIT cell recognition of antigen is restricted by MHC-related molecule 1 (MR1), a nonpolymorphic Class Ib Major Histocompatibility Complex (MHC) molecule, that associates with β2-microglobulin. MAIT cells are found in human peripheral blood, mesenteric lymph nodes, liver, and mucosa associated with the lungs and intestines. MAIT cells play a role in detecting and fighting off microbial infections. The specificity of OF-5A12 for the TCR Vα7.2 was confirmed by its ability to block the binding of another human TCR Vα7.2-specific antibody, 3C10, to MAIT cells.
566739 Rev. 1
Format Details
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
496 nm, 566 nm
576 nm
566739 Rev.1
Citations & References
View product citations for antibody "566739" on CiteAb
Development References (3)
-
Godfrey DI, Uldrich AP, McCluskey J, Rossjohn J, Moody DB. The burgeoning family of unconventional T cells.. Nat Immunol. 2015; 16(11):1114-23. (Biology). View Reference
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Gold MC, Cerri S, Smyk-Pearson S, et al. Human mucosal associated invariant T cells detect bacterially infected cells.. PLoS Biol. 2010; 8(6):e1000407. (Clone-specific). View Reference
-
Gold MC, Eid T, Smyk-Pearson S, et al. Human thymic MR1-restricted MAIT cells are innate pathogen-reactive effectors that adapt following thymic egress. Mucosal Immunol. 2013; 6(1):35-44. (Immunogen: Blocking, Flow cytometry, Fluorescence activated cell sorting). View Reference
566739 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.