PE Mouse Anti-Human TCR Cβ2

BD Pharmingen™ PE Mouse Anti-Human TCR Cβ2

Name: PE Mouse Anti-Human TCR Cβ2
Brand: BD Pharmingen™
SKU: 571190

Clone SAM.2.rMAb (also known as KFN)

(RUO)

Multiparameter flow cytometric analysis of TCR Cβ2 expression on Human peripheral blood lymphocytes. Human whole blood was stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; Left Plot), PE Mouse Anti-Human TCR Cβ2 antibody (Cat. No. 571190/571191; Middle and Right Plots) and BD OptiBuild™ BV421 Mouse Anti-Human TCR Cβ1 antibody (Cat. No. 747982; Right Plot) at 0.5 µg/test. Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The pseudocolor density plots showing the correlated expression of TCR Cβ2 (or Ig Isotype control staining) versus side light-scatter (SSC-A) signals or TCR Cβ1 were derived from gated events with the light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software. Data shown on this Technical Data Sheet are not lot specific.

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Product Details

Brand: BD Pharmingen™

Alternative Name: T cell receptor beta constant 2; TCR Cβ2; TCRBC2; TRBC2

Reactivity: Human (QC Testing)

Isotype: Mouse IgG1, κ

Application: Flow cytometry (Routinely Tested)

Concentration: 0.2 mg/ml

Entrez Gene ID: 28638

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Since applications vary, each investigator should titrate the reagent to obtain optimal results.
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
For U.S. patents that may apply, see bd.com/patents.

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Stain Buffer (BSA) RUO

Size 500 mL Cat No. 554657

PE Mouse IgG1, κ Isotype Control RUO

Size 0.1 mg Cat No. 554680

BV421 Mouse Anti-Human TCR Cβ1 RUO

Size 50 µg Cat No. 747982

Lysing Buffer RUO

Size 100 mL Cat No. 555899

Lysing Solution 10X Concentrate IVD

Size 100 mL Cat No. 349202

View Details

571190 Rev. 2

Antibody Details

SAM.2.rMAb

The SAM.2.rMAb is a recombinant monoclonal antibody that recognizes TCR Cβ2 expressed by a large proportion of CD4+ and CD8+ T cells. Thymocytes and mature peripheral T cells predominantly express a heterodimeric T cell receptor (TCR αβ) for antigen which is comprised of disulfide-liked transmembrane α and β chain subunits. The constant region of the TCR α subunit is encoded by TRAC, whereas the TCR β subunit is encoded by either of two highly homologous constant region genes, TCRB1 for TCR Cβ1 or TCRB2 for TCR Cβ2. The JOVI.1 antibody alternatively recognizes TCR Cβ1 expressed by the other TCR αβ+ T cells. These antibodies are effectively used together in multicolor staining and flow cytometric analyses to identify and characterize the natures of either TCR Cβ1+ or TCR Cβ2+ T cells in heterogeneous cell populations.

Note - Some human CD3- and TCR-specific antibodies might not be compatible to co-stain human T cells with SAM2.rMab. To confirm compatibility and immunofluorescent staining protocols, please visit: https://www.bdbiosciences.com/content/dam/bdb/marketing-documents/discover-learn/thought-leadership/events/POSTER-AAI%20202024-S-Riguad.pdf

571190 Rev. 2

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

571190 Rev.2

Citations & References

View product citations for antibody "571190" on CiteAb

Development References (7)

Berg H, Otteson GE, Corley H, et al. Flow cytometric evaluation of TRBC1 expression in tissue specimens and body fluids is a novel and specific method for assessment of T-cell clonality and diagnosis of T-cell neoplasms.. Cytometry B Clin Cytom. 2021; 100(3):361-369. (Biology). View Reference
Ferrari M, Baldan V, Ghongane P, et al. Targeting TRBC1 and 2 for the treatment of T cell lymphomas. Abstract. Cancer Res. 2020; 80:2183. (Biology).
Horna P, Shi M, Olteanu H, Johansson U. Emerging Role of T-cell Receptor Constant β Chain-1 (TRBC1) Expression in the Flow Cytometric Diagnosis of T-cell Malignancies.. Int J Mol Sci. 2021; 22(4):1817. (Biology). View Reference
Maciocia PM, Wawrzyniecka PA, Philip B, et al. Targeting the T cell receptor β-chain constant region for immunotherapy of T cell malignancies.. Nat Med. 2017; 23(12):1416-1423. (Biology). View Reference
Morath A, Schamel WW. αβ and γδ T cell receptors: Similar but different.. J Leukoc Biol. 2020; 107(6):1045-1055. (Biology). View Reference
Muñoz-García N, Lima M, Villamor N, et al. Anti-TRBC1 Antibody-Based Flow Cytometric Detection of T-Cell Clonality: Standardization of Sample Preparation and Diagnostic Implementation.. Cancers (Basel). 2021; 13(17):4379. (Biology). View Reference
Tesio M. Subtle Differences to Make the Difference.. Hemasphere. 2018; 2(2):e38. (Biology). View Reference

View All (7)

571190 Rev. 2

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.