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Enhance your research with PE-Cy7 alternative with reduced spillover and monocyte background! Try BD Horizon RealBlue™ 780 SKU# 755331 today! More Info on RB780 #
561294Image1.png

Flow cytometric analysis of CD45 expression on Rhesus macaque peripheral blood leukocytes. Rhesus macaque whole blood was stained with PE-Cy™7 Mouse anti-NHP CD45 antibody (Cat. No. 561294; Right Panel) or with a PE-Cy™7 Mouse IgG1, κ Isotype Control (Cat. No. 557872; Left Panel). The erythrocytes were lysed with BD PharmLyse™ Lysing Buffer (Cat. No. 555899). Two parameter flow cytometric dot blots showing the correlated expression of CD45 (or Ig Isotype control staining) versus side-scattered light signals were derived from events with the forward light scatter signals of viable leukocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System. 

NHP_CD45_PE-Cy7.png

Flow cytometric analysis of CD45 expression on Rhesus macaque peripheral blood leukocytes. Rhesus macaque whole blood was stained with PE-Cy™7 Mouse anti-NHP CD45 antibody (Cat. No. 561294; Right Panel) or with a PE-Cy™7 Mouse IgG1, κ Isotype Control (Cat. No. 557872; Left Panel). The erythrocytes were lysed with BD PharmLyse™ Lysing Buffer (Cat. No. 555899). Two parameter flow cytometric dot blots showing the correlated expression of CD45 (or Ig Isotype control staining) versus side-scattered light signals were derived from events with the forward light scatter signals of viable leukocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System. 

561294Image1.png NHP_CD45_PE-Cy7.png Blue-Cap-Blue-Label.png

Flow cytometric analysis of CD45 expression on Rhesus macaque peripheral blood leukocytes. Rhesus macaque whole blood was stained with PE-Cy™7 Mouse anti-NHP CD45 antibody (Cat. No. 561294; Right Panel) or with a PE-Cy™7 Mouse IgG1, κ Isotype Control (Cat. No. 557872; Left Panel). The erythrocytes were lysed with BD PharmLyse™ Lysing Buffer (Cat. No. 555899). Two parameter flow cytometric dot blots showing the correlated expression of CD45 (or Ig Isotype control staining) versus side-scattered light signals were derived from events with the forward light scatter signals of viable leukocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System. 

Flow cytometric analysis of CD45 expression on Rhesus macaque peripheral blood leukocytes. Rhesus macaque whole blood was stained with PE-Cy™7 Mouse anti-NHP CD45 antibody (Cat. No. 561294; Right Panel) or with a PE-Cy™7 Mouse IgG1, κ Isotype Control (Cat. No. 557872; Left Panel). The erythrocytes were lysed with BD PharmLyse™ Lysing Buffer (Cat. No. 555899). Two parameter flow cytometric dot blots showing the correlated expression of CD45 (or Ig Isotype control staining) versus side-scattered light signals were derived from events with the forward light scatter signals of viable leukocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System. 

Product Details
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BD Pharmingen™
Pan Leukocyte, NHP-specific; PTPRC; LCA; L-CA; Leukocyte Common Ag
Rhesus, Cynomolgus, Baboon (QC Testing)
Mouse IgG1, κ
Rhesus peripheral whole blood
Flow cytometry (Routinely Tested)
5 µl
AB_10612014
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with PE-Cy7 under optimum conditions, and unconjugated antibody and free PE-Cy7 were removed.
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Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Warning: Some APC-Cy7 and PE-Cy7 conjugates show changes in their emission spectrum with prolonged exposure to formaldehyde. If you are unable to analyze fixed samples within four hours, we recommend that you use BD™ Stabilizing Fixative (Cat. No. 338036).
  8. PE-Cy7 is a tandem fluorochrome composed of R-phycoerythrin (PE), which is excited by 488-nm light and serves as an energy donor, coupled to the cyanine dye Cy7, which acts as an energy acceptor and fluoresces maximally at 780 nm. PE-Cy7 tandem fluorochrome emission is collected in a detector for fluorescence wavelengths of 750 nm and higher. Although every effort is made to minimize the lot-to-lot variation in the efficiency of the fluorochrome energy transfer, differences in the residual emission from PE may be observed. Therefore, we recommend that individual compensation controls be performed for every PE-Cy7 conjugate. PE-Cy7 is optimized for use with a single argon ion laser emitting 488-nm light, and there is no significant overlap between PE-Cy7 and FITC emission spectra. When using dual-laser cytometers, which may directly excite both PE and Cy7, we recommend the use of cross-beam compensation during data acquisition or software compensation during data analysis.
  9. Cy is a trademark of GE Healthcare.
  10. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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561294 Rev. 2
Antibody Details
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D058-1283

D058-1283 is a CD45 monoclonal antibody specific for non-human primate leucocytes. It was developed using Rhesus peripheral whole blood as the immunogen. It does not cross-react with human leucocytes. This antibody reacts with baboon, Rhesus and Cynomolgus Macaque leucocytes in a similar pattern to CD45 binding to leukocyte common antigen (LCA) on human cells. Immunophenotypic analysis shows that D058-1283 binds to lymphocytes, monocytes and granulocytes of non-human primate blood samples. This antibody is able to block the binding of monoclonal antibody TÜ116; a reported anti-human CD45 antibody that cross-reacts with nonhuman primate leucocytes. In Western blot analysis, the D058-1283 antibody identifies a 180-200 kDa band.

561294 Rev. 2
Format Details
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PE-Cy7
PE-Cy7 dye is a part of the BD PE family of dyes. This tandem fluorochrome is comprised of a R-Phycoerythrin (PE) donor that has excitation maxima (Ex Max) of 496-nm and 566-nm and an acceptor dye, Cy™7, with an emission maximum (Em Max) at 781-nm. PE can be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and detected using an optical filter centered near 781 nm (e.g., a 760/60-nm bandpass filter). The donor dye can be excited by the Blue (488-nm), Green (532-nm) and Yellow-Green (561-nm) lasers and the acceptor dye can be excited by the Red (627–640-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE-Cy7
496 nm, 566 nm
781 nm
561294 Rev.2
Citations & References
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View product citations for antibody "561294" on CiteAb

Development References (3)

  1. Kishihara K, Penninger J, Wallace VA, et al. Normal B lymphocyte development but impaired T cell maturation in CD45-exon6 protein tyrosine phosphatase-deficient mice. Cell. 1993; 74(1):143-156. (Biology). View Reference
  2. Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
  3. Reimann KA, Waite BC, Lee-Parritz DE, et al. Use of human leukocyte-specific monoclonal antibodies for clinically immunophenotyping lymphocytes of rhesus monkeys. Cytometry. 1994; 17(1):102-108. (Biology). View Reference
561294 Rev. 2

 

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

Please refer to Support Documents for Quality Certificates

 

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

 

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.