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BD Pharmingen™ FITC Mouse Anti-Rat CD3
Clone G4.18 (RUO)
Flow cytometric analysis of CD3e expression in rat spleen and thymus. LEW splenocytes were simultaneously stained with PE Mouse Anti-Rat CD4 (Cat. No. 554838; left panels), PE Mouse Anti-Rat CD8a (Cat. No. 559976/554857; left panels) and FITC Mouse Anti-Rat CD3 (Cat. No. 554832/559975; bottom left panel) monoclonal antibodies. Please note that CD3-CD4+ monocytes and CD3- CD8a+ NK cells may be found in the rat spleen. LEW thymocytes were stained with FITC Mouse Anti-Rat CD3 (bottom right panel) or unstained (top right panel). The fluorescence contour plots/histograms were derived from gated events with the forward and side light-scattering characteristics of viable cells. Flow cytometry was performed on a BD FACScan™.
Flow cytometric analysis of CD3e expression in rat spleen and thymus. LEW splenocytes were simultaneously stained with PE Mouse Anti-Rat CD4 (Cat. No. 554838; left panels), PE Mouse Anti-Rat CD8a (Cat. No. 559976/554857; left panels) and FITC Mouse Anti-Rat CD3 (Cat. No. 554832/559975; bottom left panel) monoclonal antibodies. Please note that CD3-CD4+ monocytes and CD3- CD8a+ NK cells may be found in the rat spleen. LEW thymocytes were stained with FITC Mouse Anti-Rat CD3 (bottom right panel) or unstained (top right panel). The fluorescence contour plots/histograms were derived from gated events with the forward and side light-scattering characteristics of viable cells. Flow cytometry was performed on a BD FACScan™.
Product Details
BD Pharmingen™
CD3 Complex; T3
Rat (QC Testing)
Mouse BALB/c IgG3, κ
PHA-stimulated rat lymph node and spleen cells
Flow cytometry (Routinely Tested)
0.5 mg/ml
AB_397402
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 ML
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 ML
Cat No.
554657
PE Mouse Anti-Rat CD8a RUO
Size
0.2 mg
Cat No.
554857
FITC Mouse IgG3, κ Isotype Control RUO
Size
0.25 mg
Cat No.
559806
FITC Mouse Anti-Rat CD3 RUO
Size
0.1 mg
Cat No.
559975
PE Mouse Anti-Rat CD4 RUO
Size
0.2 mg
Cat No.
554838
554832 Rev. 18
Antibody Details
G4.18
The G4.18 monoclonal antibody specifically recognizes the T-cell receptor-associated CD3 cell-surface antigen found on thymocytes, peripheral T lymphocytes, and dendritic epidermal T cells. It has been reported that CD3 expression is down-regulated within 24 hours in concanavalin A-stimulated rat T cells, and soluble mAb inhibits the allogeneic mixed-lymphocyte proliferative response and cell-mediated cytotoxicity to allogeneic target cells. In vivo treatment with G4.18 mAb prevents cardiac and skin allograft rejection, resulting in donor-specific tolerance. Pre-incubation of splenocytes with the alternate anti-rat CD3 monoclonal antibody, 1F4, blocks staining with mAb G4.18.
554832 Rev. 18
Format Details
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
FITC
494 nm
518 nm
554832 Rev.18
Citations & References
View product citations for antibody "554832" on CiteAb
Development References (5)
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Morris DL, Komocsar WJ. Immunophenotyping analysis of peripheral blood, splenic, and thymic lymphocytes in male and female rats. J Pharmacol Toxicol Methods. 1997; 37(1):37-46. (Clone-specific). View Reference
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Nelson DJ, McMenamin C, McWilliam AS, Brenan M, Holt PG. Development of the airway intraepithelial dendritic cell network in the rat from class II major histocompatibility (Ia)-negative precursors: differential regulation of Ia expression at different levels of the respiratory tract. J Exp Med. 1994; 179(1):203-212. (Clone-specific: Immunohistochemistry). View Reference
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Nicolls MR, Aversa GG, Pearce NW, et al. Induction of long-term specific tolerance to allografts in rats by therapy with an anti-CD3-like monoclonal antibody.. Transplantation. 1993; 55(3):459-68. (Immunogen: (Co)-stimulation, Flow cytometry, Immunohistochemistry, Immunoprecipitation, Inhibition, Stimulation). View Reference
-
Strickland D, Kees UR, Holt PG. Regulation of T-cell activation in the lung: alveolar macrophages induce reversible T-cell anergy in vitro associated with inhibition of interleukin-2 receptor signal transduction. Immunology. 1996; 87(2):250-258. (Biology). View Reference
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Upham JW, Strickland DH, Bilyk N, Robinson BW, Holt PG. Alveolar macrophages from humans and rodents selectively inhibit T-cell proliferation but permit T-cell activation and cytokine secretion. Immunology. 1995; 84(1):142-147. (Biology). View Reference
554832 Rev. 18
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.