Skip to main content Skip to navigation
Hamburger Menu
Search icon
Country Selector Country Selector
Ireland (English)
Profile Icon Profile Icon
Sign-in/Register UserName
Products

Link Arrow
Solutions

Link Arrow
Discover & Learn

Link Arrow
Resources & Tools

Link Arrow
Support

Link Arrow
Search icon Search icon
Close Menu
      Alert Icon
      FITC Mouse Anti-Pig CD3ε
      FITC Mouse Anti-Pig CD3ε
      CD3ε and CD4 expression on peripheral blood leukocytes. Pig whole blood was stained with FITC-conjugated BB23-8E6-8C8 and PE- conjugated 74-12-4 (anti-pig CD4a, Cat. no. 559586) monoclonal antibodies. Erythrocytes were lysed (PharmLyse™, Cat. no. 555899), and non-viable leukocytes were excluded by staining with 7-AAD (Via-Probe™, Cat. no. 555816/555815). Flow cytometry was performed on a FACSCalibur™ (BDIS, San Jose, CA).
      FITC Mouse Anti-Pig CD3ε
      CD3ε and CD4 expression on peripheral blood leukocytes. Pig whole blood was stained with FITC-conjugated BB23-8E6-8C8 and PE- conjugated 74-12-4 (anti-pig CD4a, Cat. no. 559586) monoclonal antibodies. Erythrocytes were lysed (PharmLyse™, Cat. no. 555899), and non-viable leukocytes were excluded by staining with 7-AAD (Via-Probe™, Cat. no. 555816/555815). Flow cytometry was performed on a FACSCalibur™ (BDIS, San Jose, CA).
      Product Details
      Down Arrow Up Arrow


      BD Pharmingen™
      Pig (QC Testing)
      Mouse BALB/c IgG2a, κ
      Pig peripheral blood mononuclear cells
      Flow cytometry (Routinely Tested)
      0.5 mg/ml
      AB_2259894
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
      Show More blue down arrow Show Less blue up arrow

      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      Show More blue down arrow Show Less blue up arrow
      559582 Rev. 5
      Antibody Details
      Down Arrow Up Arrow
      BB23-8E6-8C8

      The BB23-8E6-8C8 monoclonal antibody specifically binds to the 25-kDa ε chain of the T-cell receptor-associated CD3 complex.  It recognizes all CD4+ and most CD8+ peripheral blood T lymphocytes, most thymocytes and phytohemagglutinin-stimulated blasts, and subsets of spleen and Peyer's patch lymphocytes. BB23-8E6-8C8 is a immunoglobulin isotype switch variant of the BB23-8E6 clone. This isotype-switch variant induces a proliferative response of peripheral blood mononuclear cells. The epitope recognized by BB23-8E6 mAb was designated CD3a by the Second International Swine CD Workshop.

      559582 Rev. 5
      Format Details
      Down Arrow Up Arrow
      FITC
      Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      FITC
      Blue 488 nm
      494 nm
      518 nm
      559582 Rev.5
      Citations & References
      Down Arrow Up Arrow
      View product citations for antibody "559582" on CiteAb

      Development References (2)

      1. Pescovitz MD, Book BK, Aasted B. Analyses of monoclonal antibodies reacting with porcine CD3: results from the Second International Swine CD Workshop. Vet Immunol Immunopathol. 1998; 60(3-4):261-268. (Clone-specific). View Reference
      2. Pescovitz MD, Book BK, Aasted B. Summary of workshop findings for antibodies reacting with porcine T-cells and activation antigens: results from the Second International Swine CD Workshop. Vet Immunol Immunopathol. 1998; 60(3-4):251-260. (Clone-specific). View Reference
      559582 Rev. 5

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.