FITC Mouse Anti-Human CD158b1, b2, j

BD Pharmingen™ FITC Mouse Anti-Human CD158b1, b2, j

Name: FITC Mouse Anti-Human CD158b1, b2, j
Brand: BD Pharmingen™
SKU: 556070

Clone DX27

(RUO)

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Scientific Resources

Flow cytometric analysis of CD158b1, b2, j expression on human blood lymphocytes. Whole blood was stained with FITC Mouse Anti-Human CD158b1, b2, j (Cat. No. 556070; solid line histogram) and FITC Mouse IgG2a, κ Isotype Control (Cat. No.555573, dashed line histogram). Erythrocytes were lysed with BD FACS Lysing Solution (Cat. No. 349202).The fluorescence histogram was derived from gated events with the forward and side light-scattering characteristics of viable lymphocytes. Flow cytometry was performed on a FACScan™.

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Product Details

Brand: BD Pharmingen™

Alternative Name: NKAT2/NKAT5/NKAT6; KIR2DL3/KIR2DS2/KIR2DL2

Reactivity: Human (QC Testing)

Isotype: Mouse IgG2a, κ

Application: Flow cytometry (Routinely Tested)

Vol. Per Test: 20 µl

Entrez Gene ID: 3804

RRID: AB_396339

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.

Recommended Assay Procedures

BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and CompBead to ensure that BD Comp beads are appropriate for your specific cellular application.

Product Notices

This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
An isotype control should be used at the same concentration as the antibody of interest.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Stain Buffer (BSA) RUO

Size 500 mL Cat No. 554657

Lysing Solution 10X Concentrate IVD

Size 100 mL Cat No. 349202

Lysing Buffer RUO

Size 100 mL Cat No. 555899

FITC Mouse IgG2a, κ Isotype Control RUO

Size 100 Tests Cat No. 555573

556070 Rev. 6

Antibody Details

DX27

The DX27 monoclonal antibody specifically recognizes CD158b1 (KIRDL2/NKAT6), CD158b2 (KIRDL3/NKAT2), and CD158j (KIR2DS2/NKAT5) which are members of the Killer immunoglobulin-like receptor (KIR) family within the Ig superfamily. CD158b1 and CD158j contain two (KIR2D) Ig-like extacellular domains whereas CD158b2 contains three (KIR3D) Ig-like domains. These polymorphic CD158 molecules are expressed on natural killer (NK) cells and a subset of T cells and can recognize MHC class I molecules on target cells. They serve as either inhibitory receptors (CD158b1, CD158b2) that express immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in their cytoplasmic domains or activating receptors (CD158j) that lack ITIMs.

556070 Rev. 6

Format Details

FITC

Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: FITC

Excitation Source: Blue 488 nm

Excitation Max: 494 nm

Emission Max: 518 nm

556070 Rev.6

Citations & References

Development References (2)

Bakker AB, Phillips JH, Figdor CG, Lanier LL. Killer cell inhibitory receptors for MHC class I molecules regulate lysis of melanoma cells mediated by NK cells, gamma delta T cells, and antigen-specific CTL. J Immunol. 1998; 160(11):5239-5245. (Biology). View Reference
Lanier LL, Corliss B, Phillips JH. Arousal and inhibition of human NK cells. Immunol Rev. 1997; 155:145-154. (Biology). View Reference

556070 Rev. 6

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.