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      BV480 Mouse Anti-Human CD1a
      BV480 Mouse Anti-Human CD1a
      Flow cytometric analysis of CD1a expression on human MOLT-4 cells. Cells from the human MOLT-4 (T lymphoblastic leukemia, ATCC CRL-1582) cell line were stained with either BD Horizon™ BV480 Mouse IgG1, κ Isotype Control (Cat. No. 565652; dashed line histogram) or BD Horizon™ BV480 Mouse Anti-Human CD1a antibody (Cat. No. 566147/566157; solid line histogram). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable MOLT-4 cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
      BV480 Mouse Anti-Human CD1a
      Flow cytometric analysis of CD1a expression on human MOLT-4 cells. Cells from the human MOLT-4 (T lymphoblastic leukemia, ATCC CRL-1582) cell line were stained with either BD Horizon™ BV480 Mouse IgG1, κ Isotype Control (Cat. No. 565652; dashed line histogram) or BD Horizon™ BV480 Mouse Anti-Human CD1a antibody (Cat. No. 566147/566157; solid line histogram). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable MOLT-4 cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
      Product Details
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      BD Horizon™
      CD1; T6/Leu-6; R4; T6; HTA-1; FCB6
      Human (QC Testing)
      Mouse IgG1, κ
      Flow cytometry (Routinely Tested)
      5 µl
      V 5T CD01.01
      909
      AB_2739545
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV480 under optimum conditions, and unconjugated antibody and free BD Horizon BV480 were removed.
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      Recommended Assay Procedures

      For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

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      Product Notices

      1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. BD Horizon Brilliant Violet 480 is covered by one or more of the following US patents: 8,575,303; 8,354,239.
      6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      566147 Rev. 2
      Antibody Details
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      HI149

      The HI149 monoclonal antibody specifically binds to CD1a. CD1a is a type I transmembrane glycoprotein. The 49 kDa CD1a polypeptide is associated with β2-microglobulin. CD1a is expressed on cortical thymocytes, dendritic cells and Langerhans cells. CD1a has structural similarities to the MHC class I antigen, and plays a role in antigen presentation.

      The antibody was conjugated to BD Horizon BV480 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 436-nm and Em Max at 478-nm, BD Horizon BV480 can be excited by the violet laser and detected in the BD Horizon BV510 (525/40-nm) filter set.  BV480 has less spillover into the BV605 detector and, in general, is brighter than BV510.

      566147 Rev. 2
      Format Details
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      BV480
      The BD Horizon Brilliant Violet™ 480 (BV480) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology fluorochrome has an excitation maximum (Ex Max) of 440-nm and an emission maximum (Em Max) of 479-nm. Driven by BD innovation, BV480 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 480-nm (e.g., a 525/50 bandpass filter). The increased fluorescence intensity of BV480 and narrower emission spectra, make it a good alternative for BV510 or V500. Due to its excitation profile, BV480 will also has less cross-laser excitation with the UV laser, resulting in less spillover into UV channels compared to BV510. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      BV480
      Violet 405 nm
      440 nm
      479 nm
      566147 Rev.2
      Citations & References
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      View product citations for antibody "566147" on CiteAb

      Development References (4)

      1. Calabi F, Bradbury A. The CD1 system. Tissue Antigens. 1991; 37(1):1-9. (Biology). View Reference
      2. Hanau D, Schmitt DA, Bieber T, Schmitt D, Cazenave JP. Possible mechanism of action of CD1a antigens. J Invest Dermatol. 1990; 95(5):503-505. (Biology). View Reference
      3. Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
      4. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
      View All (4) View Less
      566147 Rev. 2

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.