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BV421 Rat Anti-Human Claudin-1
BV421 Rat Anti-Human Claudin-1
Flow cytometric analysis of Claudin-1 expression on human epidermoid carcinoma cells. A431 cells (ATCC CRL-1555) were harvested using trypsin, and then stained with either BD OptiBuild™ BV421 Mouse Anti-Human Claudin-1 (Cat. No. 748199, solid line histogram) or BD Horizon™ BV421 Rat IgG2a, κ Isotype Control (Cat. No. 562602; dashed line histogram) at 1.0 µg/test. The fluorescence histogram showing Claudin-1 (or Ig Isotype control) staining was derived from gated events with the forward and side light-scatter characteristics of viable singlet cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo® software.   The above is qualification data only and does not represent a specific OptiBuild™ lot.
Flow cytometric analysis of Claudin-1 expression on human epidermoid carcinoma cells. A431 cells (ATCC CRL-1555) were harvested using trypsin, and then stained with either BD OptiBuild™ BV421 Mouse Anti-Human Claudin-1 (Cat. No. 748199, solid line histogram) or BD Horizon™ BV421 Rat IgG2a, κ Isotype Control (Cat. No. 562602; dashed line histogram) at 1.0 µg/test. The fluorescence histogram showing Claudin-1 (or Ig Isotype control) staining was derived from gated events with the forward and side light-scatter characteristics of viable singlet cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo® software.   The above is qualification data only and does not represent a specific OptiBuild™ lot.
Product Details
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BD OptiBuild™
CLD1; CLDN1; claudin-1; ILVASC; SEMP1
Human (Tested in Development)
Rat IgG2a, κ
Human Claudin-1 Transfected Cell Line
Flow cytometry (Qualified)
0.2 mg/ml
AB_2872633
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimal conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

Product Notices

  1. This antibody was developed for use in flow cytometry.
  2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  3. Researchers should determine the optimal concentration of this reagent for their individual applications.
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  9. BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
  10. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
748199 Rev. 2
Antibody Details
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421203

The 421203 monoclonal antibody specifically recognizes Claudin-1 which is also known as Senescence-associated epithelial membrane protein 1 (SEMP1) or Ichthyosis, leukocyte vacuoles, alopecia, and sclerosing cholangitis (ILVASC). Claudin-1 is a ~23 kDa multi-pass membrane protein that is encoded by CLDN1 (claudin 1) which belongs to the claudin family. Claudin-1 is expressed by Langerhans cells, dendritic cells, epithelial cells and endothelial cells. It serves as a major component of tight junctions between epithelial or endothelial cells. Claudin-1 may also function as a co-receptor for Hepatitis C virus (HCV) entry into hepatic cells. Dysregulated Claudin-1 expression has been detected in multiple types of cancer.

The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.

748199 Rev. 2
Format Details
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BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BV421
Violet 405 nm
407 nm
423 nm
748199 Rev.2
Citations & References
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View product citations for antibody "748199" on CiteAb

Development References (2)

  1. Farquhar MJ, Hu K, Harris HJ, et al. Hepatitis C virus induces CD81 and claudin-1 endocytosis.. J Virol. 2012; 86(8):4305-16. (Clone-specific: Immunocytochemistry). View Reference
  2. Furuse M, Fujita K, Hiiragi T, Fujimoto K, Tsukita S. Claudin-1 and -2: novel integral membrane proteins localizing at tight junctions with no sequence similarity to occludin.. J Cell Biol. 1998; 141(7):1539-50. (Biology). View Reference
748199 Rev. 2

 

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.