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Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Recommended Assay Procedures
BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD CompBead to ensure that BD CompBeads are appropriate for your specific cellular application.
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).
Note: When using high concentrations of antibody, background binding of this dye to erythroid cell subsets (mature erythrocytes and precursors) has been observed. For researchers studying these cell populations, or in cases where light scatter gating does not adequately exclude these cells from the analysis, this background may be an important factor to consider when selecting reagents for panel(s).
Product Notices
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- Researchers should determine the optimal concentration of this reagent for their individual applications.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- BD Horizon Brilliant Ultraviolet 661 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,575,303; 8,354,239.
Companion Products
The 10D5 monoclonal antibody specifically recognizes Integrin alpha v beta 6 (αvβ6) that is also known as CD51/β6. This heterodimeric receptor is comprised of transmembrane type I alpha and beta chain glycoproteins that are encoded by ITGAV (integrin subunit alpha) and ITGB6 (integrin subunit beta 6), respectively. Like other integrins, αvβ6 serves as a transmembrane adhesion receptor that can tether the intracellular cytoskeleton to the extracellular matrix (ECM) by binding to R-G-D sequences of ECM protein ligands including fibronectin, vitronectin, and tenascin. Integrin αvβ6 likewise plays a key role in the activation of transforming growth factor beta-1 (TGFβ1) by its R-G-D-dependent release of TGFβ1 from regulatory Latency-associated peptide (LAP). Some viruses, such as. Coxsackieviruses and Herpes simplex virus-1 use Integrin αvβ6 as a receptor to infect cells. Integrin αvβ6 engages in bidirectional signaling, ie, outside-in and inside-out signaling, to regulate essential cellular functions. These include adhesion, migration, polarization, differentiation, and proliferation that are crucial to tissue homeostasis. Although not normally expressed on adult epithelial cells/keratinocytes, its expression is upregulated on these cells during the course of embryonic development, inflammation, tissue injury, wound healing, as well as malignant transformation. The 10D5 antibody blocks Integrin αvβ6-dependent cellular adhesion and migration and reportedly recognizes both human and mouse αvβ6 integrins. Binding of 10D5 antibody to αvβ6 is divalent-cation dependent.
The antibody was conjugated to BD Horizon™ BUV661 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348-nm and an acceptor dye with an Em Max at 661-nm. BD Horizon Brilliant BUV661 can be excited by the ultraviolet laser (355 nm) and detected with a 670/25 filter and a 630 nm LP. Due to cross laser excitation of this dye, there may be significant spillover into channels detecting APC-like emissions (eg, 670/25-nm filter).
Due to spectral differences between labeled cells and beads, using BD™ CompBeads can result in incorrect spillover values when used with BD Horizon BUV661 reagents. Therefore, the use of BD CompBeads or BD CompBeads Plus to determine spillover values for these reagents is not recommended. Different BUV661 reagents (eg, CD4 vs. CD45) can have slightly different fluorescence spillover therefore, it may also be necessary to use clone-specific compensation controls when using these reagents.
Development References (14)
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Allen MD, Thomas GJ, Clark S, et al. Altered microenvironment promotes progression of preinvasive breast cancer: myoepithelial expression of αvβ6 integrin in DCIS identifies high-risk patients and predicts recurrence.. Clin Cancer Res. 2014; 20(2):344-57. (Clone-specific: Blocking, Flow cytometry, Functional assay). View Reference
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Bandyopadhyay A, Raghavan S. Defining the role of integrin alphavbeta6 in cancer.. Curr Drug Targets. 2009; 10(7):645-52. (Biology). View Reference
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Bates RC, Bellovin DI, Brown C, et al. Transcriptional activation of integrin beta6 during the epithelial-mesenchymal transition defines a novel prognostic indicator of aggressive colon carcinoma. J Clin Invest. 2005; 115(2):339-47. (Clone-specific: Blocking). View Reference
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Huang X, Wu J, Spong S, Sheppard D. The integrin alphavbeta6 is critical for keratinocyte migration on both its known ligand, fibronectin, and on vitronectin. J Cell Sci. 1998; 111(15):2189-95. (Immunogen: Flow cytometry, Functional assay, Immunoprecipitation, Inhibition). View Reference
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Jackson T, Sheppard D, Denyer M, Blakemore W, King AM. The epithelial integrin αvβ6 is a receptor for foot-and-mouth disease virus. J Virol. 2000; 74(11):4949-56. (Clone-specific: Blocking). View Reference
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Jovanovic J, Takagi J, Choulier L, et al. alphaVbeta6 is a novel receptor for human fibrillin-1. Comparative studies of molecular determinants underlying integrin-rgd affinity and specificity.. J Biol Chem. 2007; 282(9):6743-51. (Clone-specific: Blocking, Flow cytometry). View Reference
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Koivisto L, Bi J, Häkkinen L, Larjava H. Integrin αvβ6: Structure, function and role in health and disease.. Int J Biochem Cell Biol. 2018; 99:186-196. (Biology). View Reference
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Li X, Yang Y, Hu Y, et al. Alphavbeta6-Fyn signaling promotes oral cancer progression.. J Biol Chem. 2003; 278(43):41646-53. (Clone-specific: Blocking). View Reference
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Maier S, Paulsson M, Hartmann U. The widely expressed extracellular matrix protein SMOC-2 promotes keratinocyte attachment and migration.. Exp Cell Res. 2008; 314(13):2477-87. (Clone-specific: Blocking). View Reference
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Miller LC, Blakemore W, Sheppard D, Atakilit A, King AM, Jackson T. Role of the cytoplasmic domain of the beta-subunit of integrin alpha(v)beta6 in infection by foot-and-mouth disease virus. J Virol. 2001; 75(9):4158-64. (Clone-specific: Blocking). View Reference
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Munger JS, Huang X, Kawakatsu H, et al. The integrin alpha v beta 6 binds and activates latent TGF beta 1: a mechanism for regulating pulmonary inflammation and fibrosis.. Cell. 1999; 96(3):319-28. (Clone-specific: Blocking, Functional assay). View Reference
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Thomas GJ, Lewis MP, Whawell SA, et al. Expression of the alphavbeta6 integrin promotes migration and invasion in squamous carcinoma cells.. J Invest Dermatol. 2001; 117(1):67-73. (Clone-specific: Blocking). View Reference
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Thomas GJ, Nyström ML, Marshall JF. Alphavbeta6 integrin in wound healing and cancer of the oral cavity.. J Oral Pathol Med. 2006; 35(1):1-10. (Biology). View Reference
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Williams CH, Kajander T, Hyypiä T, Jackson T, Sheppard D, Stanway G. Integrin alpha v beta 6 is an RGD-dependent receptor for coxsackievirus A9. J Virol. 2004; 78(13):6967-73. (Clone-specific). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.