Skip to main content Skip to navigation
BUV395 Rat Anti-Mouse Siglec-E
Product Details
Down Arrow Up Arrow


BD OptiBuild™
Siglec12; Siglece; mSiglec-E; myeloid inhibitory siglec; siglec-12; siglec-E
Mouse (Tested in Development)
Rat IgG2b
Mouse Siglec-E Recombinant Protein
Flow cytometry (Qualified)
0.2 mg/ml
AB_2872614
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BUV395 under optimal conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

Product Notices

  1. This antibody was developed for use in flow cytometry.
  2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  3. Researchers should determine the optimal concentration of this reagent for their individual applications.
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  9. BD Horizon Brilliant Ultraviolet 395 is covered by one or more of the following US patents: 8,158,444; 8,575,303; 8,354,239.
748153 Rev. 3
Antibody Details
Down Arrow Up Arrow
750620

The 750620 monoclonal antibody specifically recognizes Sialic acid binding Ig-like lectin E (Siglec-E).  Siglec-E is a type I transmembrane glycoprotein that is encoded by Siglece which belongs to the CD33-related subfamily of Siglecs within the immunoglobulin gene superfamily (IgSF). The extracellular region of Siglec-E contains an N-terminal V-type Ig domain followed by a C2 type 1 and C2 type 2 domain whereas the intracellular region has an immunoreceptor tyrosine-based inhibitory motif (ITIM) followed by a SLAM-like motif. Siglec-E mediates sialic-acid dependent binding to cells. Siglec-E is expressed on neutrophils and monocytes and can function as an inhibitory receptor which regulates the recruitment and effector responses of these cells.

The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry. With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter.

748153 Rev. 3
Format Details
Down Arrow Up Arrow
BUV395
The BD Horizon Brilliant™ Ultraviolet 395 (BUV395) Dye is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This base dye is a polymer fluorochrome with an excitation maximum (Ex Max) of 348-nm and an emission maximum (Em Max) at 395-nm. Driven by BD innovation, BUV395 is designed to be excited by the ultraviolet laser (355-nm) and detected using an optical filter centered near 380-nm (e.g., 379/28-nm bandpass filter). BUV395 is the ideal dye when using only one detector on the ultraviolet laser as it spills into no other detectors and no other fluors spill into it. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
BUV395
Ultraviolet 355 nm
348 nm
395 nm
748153 Rev.3
Citations & References
Down Arrow Up Arrow
View product citations for antibody "748153" on CiteAb

Development References (2)

  1. Siddiqui S, Schwarz F, Springer S, et al. Studies on the Detection, Expression, Glycosylation, Dimerization, and Ligand Binding Properties of Mouse Siglec-E. J Biol Chem. 2017; 292(3):1029-1037. (Biology). View Reference
  2. Wu Y, Ren D, Chen GY. Siglec-E Negatively Regulates the Activation of TLR4 by Controlling Its Endocytosis.. J Immunol. 2016; 197(8):3336-3347. (Clone-specific: Flow cytometry, Immunofluorescence). View Reference
748153 Rev. 3

 

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.