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APC Mouse Anti-Human TCR γδ
APC Mouse Anti-Human TCR γδ
Flow cytometric analysis of TCR γδ expression on human peripheral blood lymphocytes. Human whole blood was costained with FITC Mouse Anti-Human CD3 (Cat. No. 561807/561806/555332/555916) and APC Mouse Anti-Human TCR γδ (Cat. No. 555718/561049). Erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). Two color dot-plots were derived from gated events with the side and forward light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was carried out on a BD FACScan™ System.
Flow cytometric analysis of TCR γδ expression on human peripheral blood lymphocytes. Human whole blood was costained with FITC Mouse Anti-Human CD3 (Cat. No. 561807/561806/555332/555916) and APC Mouse Anti-Human TCR γδ (Cat. No. 555718/561049). Erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). Two color dot-plots were derived from gated events with the side and forward light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was carried out on a BD FACScan™ System.
Product Details
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BD Pharmingen™
TCRgd; γδ TCR; TRG@, TRD@; TCRG, TCRD; TCR gamma delta
Human (QC Testing)
Mouse IgG1, κ
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_398611
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
555718 Rev. 11
Antibody Details
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B1

The B1 monoclonal antibody specifically binds to the γδ T cell receptor (γδ TCR). This receptor complex consists of two disulfide-linked transmembrane glycoproteins, a γ chain (45-60 kDa) and a δ subunit (40-60 kDa). The γδ TCR is associated with the signal-transducing CD3 complex. The γδ TCR is expressed by thymocytes and by peripheral T cell subsets (γδ T cells) that are located in the blood, liver, skin and various lymphoid and mucosal tissues. γδ T cells contribute to both innate and adaptive immune responses to infections and tumors. Reports suggest that γδ T cells may also play roles in antigen presentation and the regulation of autoimmune responses.

555718 Rev. 11
Format Details
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APC
Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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APC
Red 627-640 nm
651 nm
660 nm
555718 Rev.11
Citations & References
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View product citations for antibody "555718" on CiteAb

Development References (10)

  1. Bai L, Picard D, Anderson B, et al. The majority of CD1d-sulfatide-specific T cells in human blood use a semiinvariant Vδ1 TCR.. Eur J Immunol. 2012; 42(9):2505-10. (Clone-specific). View Reference
  2. Barclay NA, Brown MH, Birkeland ML, et al, ed. The Leukocyte Antigen FactsBook. San Diego, CA: Academic Press; 1997.
  3. Bonneville M, O'Brien RL, Born WK. Gammadelta T cell effector functions: a blend of innate programming and acquired plasticity. Nat Rev Immunol. 2110; 10(7):467-478. (Biology). View Reference
  4. Breit TM, Wolvers-Tettero IL, van Dongen JJ. Receptor diversity of human T-cell receptor gamma delta expressing cells. Prog Histochem Cytochem. 1992; 26(1-4):182-193. (Biology). View Reference
  5. Davodeau F, Peyrat MA, Houde I, et al. Surface expression of two distinct functional antigen receptors on human gamma delta T cells. Science. 1993; 260(5115):1800-1802. (Biology). View Reference
  6. De Libero G. Sentinel function of broadly reactive human gamma delta T cells. Immunol Today. 1997; 18(1):22-26. (Biology). View Reference
  7. Fitzgerald KA, Callard RE. The cytokine factsbook., 2nd ed. / Katherine A. Fitzgerald ... [et al.].. San Diego: Academic Press; 2001:1-515.
  8. Kabelitz D, Pechhold K, Bender A, et al. Activation and activation-driven death of human gamma/delta T cells. Immunol Rev. 1991; 120:71-88. (Biology). View Reference
  9. Kabelitz D. Function and specificity of human gamma/delta-positive T cells. Crit Rev Immunol. 1992; 11(5):281-303. (Biology). View Reference
  10. Kabelitz D. γδ T-cells: cross-talk between innate and adaptive immunity. Cell Mol Life Sci. 2011; 68(14):2331-2333. (Biology). View Reference
View All (10) View Less
555718 Rev. 11

 

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