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Fix Buffer I

BD Phosflow™ Fix Buffer I

Product Details
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BD Phosflow™ Fix Buffer I can be used for simultaneous fixation and permeabilization of cells prior to intracellular staining. The product contains 250 ml of a 1X buffer solution.

Recommended Assay Procedures

BD Phosflow™ Fix Buffer I can be used to fix and permeabilize cells for subsequent immunofluorescent staining of intracellular proteins, and is optimized for use with the BD Phosflow brand of phosphorylated intracellular signaling protein-specific antibodies.

Danger:  BD Phosflow™ Fix Buffer I contains 4.2% formaldehyde (w/w).

Hazard statements

Causes skin irritation.

Causes serious eye damage.

May cause an allergic skin reaction.

Suspected of causing genetic defects.

May cause cancer.

Precautionary statements

Wear protective clothing / eye protection.Wear protective gloves.Use personal protective equipment as required.

Contaminated work clothing must not be allowed out of the workplace.

IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.

If skin irritation or rash occurs: Get medical advice/attention.

IF ON SKIN: Wash with plenty of water. Immediately call a POISON CENTER/doctor.

Wash thoroughly after handling.

Obtain special instructions before use. Do not handle until all safety precautions have been read and understood.

Avoid release to the environment.

Store locked up.  Dispose of contents/container to an appropriate treatment and disposal facility in accordance with applicable laws and regulations, and product characteristics at time of disposal.

Product Notices

  1. Please refer to for technical protocols.
557870 Rev. 4
Citations & References
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Development References (6)

  1. Assenmacher M, Schmitz J, Radbruch A. Flow cytometric determination of cytokines in activated murine T helper lymphocytes: expression of interleukin-10 in interferon-gamma and in interleukin-4-expressing cells. Eur J Immunol. 1994; 24(5):1097-1101. (Biology). View Reference
  2. Elson LH, Nutman TB, Metcalfe DD, Prussin C. Flow cytometric analysis for cytokine production identifies T helper 1, T helper 2, and T helper 0 cells within the human CD4+CD27- lymphocyte subpopulation. J Immunol. 1995; 154(9):4294-4301. (Biology). View Reference
  3. Jung T, Schauer U, Heusser C, Neumann C, Rieger C. Detection of intracellular cytokines by flow cytometry. J Immunol Methods. 1993; 159(1-2):197-207. (Biology). View Reference
  4. Krutzik PO, Nolan GP. Intracellular phospho-protein staining techniques for flow cytometry: monitoring single cell signaling events. Cytometry A. 2003; 55(2):61-70. (Biology). View Reference
  5. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Biology). View Reference
  6. Sander B, Andersson J, Andersson U. Assessment of cytokines by immunofluorescence and the paraformaldehyde-saponin procedure. Immunol Rev. 1991; 119:65-93. (Biology). View Reference
View All (6) View Less
557870 Rev. 4

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.