PE Mouse Anti-Human CD20 PE

BD Quantibrite™ PE Mouse Anti-Human CD20 PE

Name: PE Mouse Anti-Human CD20 PE
Brand: BD Quantibrite™
SKU: 347201

Clone L27

(RUO (GMP))

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Product Details

Brand: BD Quantibrite™

Alternative Name: MS4A1; Membrane-spanning 4-domains subfamily A member 1; B1; Bp35; LEU-16

Reactivity: Human

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Human Lymphoblastoid Cell Line

Application: Flow cytometry

Concentration: 100 μg/mL

Vol. Per Test: 20 μL

Workshop Number: III B 006

Entrez Gene ID: 931

Storage Buffer: Phosphate buffered saline with BSA and 0.1% sodium azide.

Regulatory Status: RUO (GMP)

Preparation And Storage

Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

347201 Rev. 1

Antibody Details

L27

The CD20 antibody, clone L27, is derived from the hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with the LB lymphoblastoid cell line.

The CD20 antibody recognizes a phosphoprotein with a molecular weight of 35 or 37 kilodaltons (kDa), depending on the degree of phosphorylation. The antigen is not glycosylated.

347201 Rev. 1

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

347201 Rev.1

Citations & References

View product citations for antibody "347201" on CiteAb

Development References (8)

Abts H, Emmerich M, Miltenyi S, Radbruch A, Tesch H. CD20 positive human B lymphocytes separated with the magnetic cell sorter (MACS) can be induced to proliferation and antibody secretion in vitro. J Immunol Methods. 1989; 125:19-28. (Biology).
Clark EA, Shu G, Ledbetter JA. Role of the Bp35 cell surface polypeptide in human B-cell activation. Proc Natl Acad Sci USA. 1985; 82:1766-1770. (Biology).
D'Arena G, Musto P, Dell'Olio M, Di Renzo N, Carotenuto M. Quantitative ﬂow cytometry for the differential diagnosis of leukemic B-cell chronic lymphoproliferative disorders. Am J Hematol. 2000; 64:275-281. (Biology).
Dörken B, Möller P, Pezzutto A, Schwartz-Albiez R, Moldenhauer G. KnappW, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York,NY: Oxford University Press; 1989:46-48.
Gopal AK, Press OW. Clinical applications of Anti-CD20 antibodies. J Lab Clin Med. 1999; 134:445-450. (Biology).
Hultin LE, Hausner MA, Hultin PM, Giorgi JV. CD20 (pan-B cell) antigen is expressed at a low level on asubpopulation of human T lymphocytes. Cytometry. 1993; 14:196-204. (Biology).
Loken MR, Shah VO, Dattilio KL, Civin CI. Flow cytometric analysis of human bone marrow. II. Normal B lymphocyte development. Blood. 1987; 70(5):1316-1324. (Biology). View Reference
Marti GE, Faguet G, Bertin P, et al. CD20 and CD5 expression in B-chronic lymphocytic leukemia. Ann NY Acad Sci. 1992; 651:480-483. (Biology).

View All (8)

347201 Rev. 1

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