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BD Quantibrite™ Anti-Human HLA-DR PE/Monocyte PerCP-Cy™5.5
(RUO (GMP))Anti-Human HLA-DR PE/Monocyte PerCP-Cy™5.5
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Description
Anti–HLA-DR, clone L243, is derived from the hybridization of mouse NS-1/1-Ag4 myeloma cells with spleen cells from BALB/c mice immunized with the human lymphoblastoid B-cell line RPMI 8866. CD14, clone MφP9, is derived from hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with peripheral blood monocytes from a patient with rheumatoid arthritis. Anti–HLA-DR recognizes a human class II major histocompatibility complex (MHC) antigen. The antigen is a transmembrane glycoprotein composed of α and β subunits that have molecular weights of 36 and 27 kDaltons (kDa), respectively. Anti–HLA-DR reacts with a nonpolymorphic HLA-DR epitope and does not cross-react with HLA-DQ or HLA-DP molecules. Anti-Monocyte PerCP-Cy5.5 reagent recognizes monocytes using two discrete specificities. This reagent includes CD14, a human monocyte/macrophage antigen, Mr 55 kDa, that recognizes the majority of the monocyte population. In addition, Anti-Monocyte PerCP-Cy5.5 recognizes FcR1 (CD64) by taking advantage of the unique recognition of CD64 by cyanine dyes. Anti-Monocyte PerCP-Cy5.5 is specially formulated to take advantage of this property of the cyanine dye to ensure detection of all monocytes (CD14 brightly positive and weakly positive) in a sample.
Preparation And Storage
The Anti–HLA-DR/Anti-Monocyte PerCP-Cy5.5 reagent is supplied as a combination of Anti–HLA-DR PE (≥95% 1:1 PE:mAb ratio) and Anti-Monocyte PerCP-Cy5.5 in a buffered gelatin solution containing an HLA-DR stabilizing reagent and 0.1% sodium azide. Store vials in the dark at 2° to 8°C. Do not freeze QuantiBRITE reagents; protect them from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.
Description | Clone | Isotype | EntrezGene ID |
---|---|---|---|
HLA-DR 1 1 RPE | N/A | N/A | N/A |
CD14 PerCP-Cy5.5 | N/A | N/A | N/A |
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Although not required, these products are manufactured in accordance with Good Manufacturing Practices.