At BD, we provide innovative instruments, reagents and software to support scientists throughout the full continuum of flow cytometry experiments.

On this page you will find helpful tools and resources to support multicolor panel design, workflow and experimental analysis.

NEW - Flow Cytometry Panel Design Quick Reference Guide

Defining the Objective of Your Experiment

This video is the first in the “Scientist to Scientist” panel design educational series, designed provide scientists tips & tricks to overcome common flow cytometry panel design challenges.

Knowing the Biology of Your Experiment

Watch our latest “Scientist to Scientist” series video to learn some fundamental strategies to improve your flow cytometry panel design.

Understanding Your Flow Cytometry Instrument’s Capabilities for Optimal Panel Design

In this video you will learn how to maximize your flow cytometry instrument’s capabilities for optimized panel design.

Flow Cytometry Instrument Characterization and Set Up for Optimal Panel Design

This video explains how to leverage flow cytometry configurations to help maximize resolution of populations of interest.

Matching Antigens & Fluorochromes for Flow Cytometry Experiments

This video explores practical strategies for matching antigens and fluorochromes for optimal flow cytometry panel design.

Avoiding Resolution Loss When Using Fix and Permeabilization Buffers for Intercellular Staining

In this video you will learn helpful strategies to help prevent loss of resolution due to intracellular fixation & permeabilization buffers.

Understanding and Managing Fluorescence Spillover

In this video you will learn how to manage fluorescence spillover to get the most out of your flow cytometry experiments.

Relationship Between Fluorescence Spillover and Spread

This video will help you understand the relationship between florescence spillover & spread to help you implement the right strategies to resolve your populations of interest.

Minimizing the Impact of Spread in Flow Cytometry Experiments

This video provides strategies to help minimize the impact of spread in flow cytometry experiments to help improve resolution.

Panel Design Workflow for Optimal Fluorochrome-antigen Matching

This video provides simple strategies for fluorochrome-antigen matching to help prevent the introduction of spread and subsequent resolution loss.

BD Horizon Brilliant™ Ultraviolet 615 Reagent

The BD Horizon Brilliant™ Ultraviolet 615 Reagents (BUV615) dye was developed exclusively by BD Biosciences, the leading innovator of flow cytometry instruments, reagents and software, using polymer technology to provide stable, high quality reagents that are bright with minimal spillover.

BD OptiBuild ^TM On-demand Reagents Video

BD OptiBuild™ On-demand reagents offer more fluorochrome options with the antibodies you need. Whether you want to minimize compensation or add new markers to complex experiments, BD OptiBuild reagents provide flexibility to evaluate new colors and simplify your panel design.

BD OptiBuild ^TM On-demand Reagents Titration Video

This video provides a brief tutorial on how to properly titrate BD OptiBuild™ On-Demand Reagents.