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Fluorochrome Faceoff
Watch your favorite fluorochromes take on the competition!
Game 3: Photostability
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Fluorescent dyes can undergo photobleaching (dimming) when exposed to light. This can cause a decrease in resolution. Additionally, some dyes may undergo spectral changes when exposed to light. In this Fluorochrome Faceoff, we evaluate the impact of light on fluorochromes that are primarily excited by the yellow-green laser with peak emission around 700 nm.
Winner’s Circle
Exposure to light can cause changes in a fluorochrome's emission profile, measured here as changes in spillover into other detectors. In an experimental setting, this can impact researchers as differences in spectral profile between reference controls and samples can lead to compensation or unmixing errors and data artifacts. This example shows changes in spillover in several detectors after 2 hours of light exposure, with SBY720 being the fluorochrome impacted the most. SBY720 also exhibited the greatest loss in resolution after light exposure.
Our winner for this Fluorochrome Faceoff is RY703 because this fluorochrome exhibited the least amount of spillover change and MFI loss across the dyes tested in this game.
PRO TIP: Keep your tubes protected from light during sample acquisition to prevent changes in spectral profiles over time.
Try the Winning Fluorochrome
Request a sample of RY703
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Disclaimer: Results and conclusions shown throughout the Fluorochrome Faceoff are based on experiments performed under the conditions described. Users should evaluate reagents with their specific protocols as results may vary with different experimental conditions.
Samples available in participating countries only. Cannot be combined with any other offer. Valid once per user. Offer valid on 25 test/ug size only. There are a limited number of samples available, and BD retains the right to cancel this offer at any time.
BD flow cytometers are Class 1 Laser Products. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
CF is a trademark of Biotium, Inc. Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.