Description
This kit provides the necessary reagents to prepare single-cell full-length Mouse TCR and BCR libraries that are compatible with both BD Rhapsody™ Whole Transcriptome Analysis (WTA) and BD Rhapsody™ Targeted mRNA assays. In addition to this kit, the following items are required:
• An mRNA library preparation kit (either the BD Rhapsody™ Whole Transcriptome Analysis [WTA] Amplification Kit [Catalog No. 633801] or the BD Rhapsody™ Targeted mRNA and AbSeq Amplification Kit [Catalog No. 633774])
• A targeted primer panel, such as the BD Rhapsody™ Immune Response Panel Mm (Catalog No. 633753)
• The BD Rhapsody™ Enhanced Cartridge Reagent Kit v3 (Catalog No. 667052)
• A BD Rhapsody™ cartridge (Catalog No. 633733 or Catalog No. 666262)
• The BD Rhapsody™ cDNA Kit (Catalog No. 633773)
Learn more about the intended use of this kit from the associated protocols for the BD Rhapsody™ Mouse TCR/BCR Next Multiomic Assay.
Preparation And Storage
Store the reagents at –25 °C to –15 °C. Before using reagents, see the BD Rhapsody™ Single-Cell Analysis System Instrument User Guide or the BD Rhapsody™ Express Single-Cell Analysis System Instrument User Guide
Product Notices
- For U.S. patents that may apply, see bd.com/patents.
| Description | Quantity/Size | Part Number | EntrezGene ID |
|---|---|---|---|
| TSO Next | 1 Each (1 ea) | 91-1295 | N/A |
| 1M MgCl2 | 1 Each (1 ea) | 91-1198 | N/A |
| Hybridization Buffer | 1 Each (4 ea) | 91-1199 | N/A |
| Mouse TCR N1 Primer | 1 Each (1 ea) | 91-1212 | N/A |
| Mouse TCR N2 Primer | 1 Each (1 ea) | 91-1213 | N/A |
| Mouse BCR N1 Primer | 1 Each (1 ea) | 91-1214 | N/A |
| Mouse BCR N2 Primer | 1 Each (1 ea) | 91-1215 | N/A |
| TCR/BCR Universal Oligo N1 | 1 Each (1 ea) | 91-1204 | N/A |
| TCR/BCR Universal Oligo N2 | 1 Each (1 ea) | 91-1205 | N/A |
| TCR/BCR Extension Buffer | 1 Each (1 ea) | 91-1206 | N/A |
| TCR/BCR Extension Enzyme | 1 Each (1 ea) | 91-1207 | N/A |
| TCR/BCR Extension Primers | 1 Each (1 ea) | 91-1208 | N/A |
| Nuclease-Free Water | 1 Each (2 ea) | 650000076 | N/A |
| Bead RT/PCR Enhancer | 1 Each (1 ea) | 91-1082 | N/A |
| PCR MasterMix | 1 Each (1 ea) | 91-1083 | N/A |
| Elution Buffer | 1 Each (2 ea) | 91-1084 | N/A |
| dNTP | 1 Each (2 ea) | 650000077 | N/A |
Development References (2)
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Centers for Disease Control and Prevention. 2007 Guideline for Isolation Precautions: Preventing Transmission of Infectious Agents in Healthcare Settings. Available: https://www.cdc.gov/infectioncontrol/guidelines/isolation/index.html March 12, 2019. View Reference
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Clinical and Laboratory Standards Institute. Protection of Laboratory Workers from Occupationally Acquired Infections; Approved Guideline- Fourth Edition. Wayne, PA: Clinical and Laboratory Standards Institute; 2014:CLSI document M29-A4.
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.