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01711D_553308_image2.png

Flow cytometric analysis of CD11b expression on mouse bone-marrow myeloid cells.  BALB/c bone-marrow leukocytes were stained with either Purified Rat IgG2b, κ Isotype Control (Cat. No. 553986; left panel) or Purified Rat Anti-CD11b antibody (Cat. No. 553308/550282/557394; right panel), followed by FITC Goat Anti-Rat Ig (Cat. No. 554016). Please note that the population of cells having the lowest SSC (erythroid and lymphoid cells) show little expression of CD11b, while cells with moderate-to-high SSC (myeloid cells) are almost uniformly CD11b positive (right panel). The contour plots showing CD11b expression (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of myeloid cells (ie, moderate-to-high side light-scatter-gated events). Flow cytometry was performed on a BD FACScan™ flow cytometry system.

553308Image1.png

Flow cytometric analysis of CD11b expression on mouse bone-marrow myeloid cells.  BALB/c bone-marrow leukocytes were stained with either Purified Rat IgG2b, κ Isotype Control (Cat. No. 553986; left panel) or Purified Rat Anti-CD11b antibody (Cat. No. 553308/550282/557394; right panel), followed by FITC Goat Anti-Rat Ig (Cat. No. 554016). Please note that the population of cells having the lowest SSC (erythroid and lymphoid cells) show little expression of CD11b, while cells with moderate-to-high SSC (myeloid cells) are almost uniformly CD11b positive (right panel). The contour plots showing CD11b expression (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of myeloid cells (ie, moderate-to-high side light-scatter-gated events). Flow cytometry was performed on a BD FACScan™ flow cytometry system.

01711D_553308_image2.png 553308Image1.png Blue-Cap-White-Label.jpg

Flow cytometric analysis of CD11b expression on mouse bone-marrow myeloid cells.  BALB/c bone-marrow leukocytes were stained with either Purified Rat IgG2b, κ Isotype Control (Cat. No. 553986; left panel) or Purified Rat Anti-CD11b antibody (Cat. No. 553308/550282/557394; right panel), followed by FITC Goat Anti-Rat Ig (Cat. No. 554016). Please note that the population of cells having the lowest SSC (erythroid and lymphoid cells) show little expression of CD11b, while cells with moderate-to-high SSC (myeloid cells) are almost uniformly CD11b positive (right panel). The contour plots showing CD11b expression (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of myeloid cells (ie, moderate-to-high side light-scatter-gated events). Flow cytometry was performed on a BD FACScan™ flow cytometry system.

Flow cytometric analysis of CD11b expression on mouse bone-marrow myeloid cells.  BALB/c bone-marrow leukocytes were stained with either Purified Rat IgG2b, κ Isotype Control (Cat. No. 553986; left panel) or Purified Rat Anti-CD11b antibody (Cat. No. 553308/550282/557394; right panel), followed by FITC Goat Anti-Rat Ig (Cat. No. 554016). Please note that the population of cells having the lowest SSC (erythroid and lymphoid cells) show little expression of CD11b, while cells with moderate-to-high SSC (myeloid cells) are almost uniformly CD11b positive (right panel). The contour plots showing CD11b expression (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of myeloid cells (ie, moderate-to-high side light-scatter-gated events). Flow cytometry was performed on a BD FACScan™ flow cytometry system.

Product Details
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BD Pharmingen™
Itgam; Integrin alpha-M; Ly-40; Mac-1a; Mac-1 alpha; CR3A; CR-3 alpha chain
Mouse (QC Testing), Human (Tested in Development)
Rat DA, also known as DA/HA IgG2b, κ
Mouse Splenic Cells
Flow cytometry (Routinely Tested), Blocking, Immunohistochemistry-frozen, Immunoprecipitation (Reported), Immunohistochemistry-paraffin, Western blot (Not Recommended)
0.5 mg/ml
AB_394772
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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557394 Rev. 19
Antibody Details
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M1/70

The M1/70 monoclonal antibody specifically binds to CD11b, also known as Integrin alpha M (Itgam or αM). CD11b is a 170-kDa type 1 transmembrane glycoprotein and belongs to the Integrin alpha chain family. CD11b serves as the alpha chain of the heterodimeric Mac-1 integrin (CD11b/CD18, αMβ2), also known as complement receptor 3 (CR3). Mac-1 mediates adhesion to ICAM-1 (CD54), ICAM-2 (CD102), fibrinogen and binding to C3bi.  Mac-1 is expressed at varying levels on granulocytes, macrophages, myeloid-derived dendritic cells, natural killer cells, microglia, and B-1 B lymphocytes.  Mac-1 expression is rapidly upregulated on neutrophils after activation, in the same time period that CD62L (L-selectin) is shed from the cell surface.  The M1/70 antibody reportedly blocks cell adherence and C3bi binding but does not block cell-mediated lysis.  Cross-reaction of the M1/70 antibody with CD11b expressed on human monocytes, polymorphonuclear leukocytes, and NK cells has been reported.

557394 Rev. 19
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
557394 Rev.19
Citations & References
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View product citations for antibody "557394" on CiteAb

Development References (11)

  1. Ault KA, Springer TA. Cross-reaction of a rat-anti-mouse phagocyte-specific monoclonal antibody (anti-Mac-1) with human monocytes and natural killer cells. J Immunol. 1981; 126(1):359-364. (Clone-specific). View Reference
  2. Beller DI, Springer TA, Schreiber RD. Anti-Mac-1 selectively inhibits the mouse and human type three complement receptor. J Exp Med. 1982; 156(4):1000-1009. (Biology: Blocking). View Reference
  3. Flotte TJ, Springer TA, Thorbecke GJ. Dendritic cell and macrophage staining by monoclonal antibodies in tissue sections and epidermal sheets. Am J Pathol. 1983; 111(1):112-124. (Clone-specific: Immunohistochemistry). View Reference
  4. Gyetko MR, Sud S, Kendall T, Fuller JA, Newstead MW, Standiford TJ. Urokinase receptor-deficient mice have impaired neutrophil recruitment in response to pulmonary Pseudomonas aeruginosa infection. J Immunol. 2000; 165(3):1513-1519. (Biology: Blocking). View Reference
  5. Kishimoto TK, Jutila MA, Berg EL, Butcher EC. Neutrophil Mac-1 and MEL-14 adhesion proteins inversely regulated by chemotactic factors. Science. 1989; 245(4923):1238-1241. (Biology). View Reference
  6. Lagasse E, Weissman IL. Flow cytometric identification of murine neutrophils and monocytes. J Immunol Methods. 1996; 197(1-2):139-150. (Methodology: Flow cytometry). View Reference
  7. Lub M, van Kooyk Y, Figdor CG. Competition between lymphocyte function-associated antigen 1 (CD11a/CD18) and Mac-1 (CD11b/CD18) for binding to intercellular adhesion molecule-1 (CD54). J Leukoc Biol. 1996; 59(5):648-655. (Biology: Immunoprecipitation). View Reference
  8. Sanchez-Madrid F, Simon P, Thompson S, Springer TA. Mapping of antigenic and functional epitopes on the alpha- and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1. J Exp Med. 1983; 158(2):586-602. (Biology: Blocking, Immunoprecipitation, Western blot). View Reference
  9. Springer T, Galfre G, Secher DS, Milstein C. Mac-1: a macrophage differentiation antigen identified by monoclonal antibody. Eur J Immunol. 1979; 9(4):301-306. (Clone-specific: Immunoprecipitation). View Reference
  10. Springer T, Galfre G, Secher DS, Milstein C. Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens. Eur J Immunol. 1978; 8(8):539-551. (Immunogen: Immunoprecipitation). View Reference
  11. Springer TA, Davignon D, Ho MK, Kurzinger K, Martz E, Sanchez-Madrid F. LFA-1 and Lyt-2,3, molecules associated with T lymphocyte-mediated killing; and Mac-1, an LFA-1 homologue associated with complement receptor function. Immunol Rev. 1982; 68:171-195. (Biology: Blocking). View Reference
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557394 Rev. 19

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

 

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.